ANNUAL REPORT
Calendar Year 2008
1. NRSP-6: UNITED STATES POTATO GENEBANK
Introduction, Classification, Preservation, Evaluation and Distribution of tuber-bearing Solanum Species.
2. COOPERATIVE AGENCIES AND PRINCIPAL
LEADERS
|
State
Agricultural Experimental Stations |
Representative |
|
|
|
|
Technical Representatives |
|
|
|
|
|
Southern Region |
J. C. Miller, Jr. |
|
Western Region Vice Chair (2009) |
I. Vales |
|
North Central Region Chairman (2009) |
D. S. Douches |
|
Northeastern Region Secretary (2009) |
W. De Jong |
|
|
|
Administrative Advisors
|
|
|
|
Southern Region |
R. Guthrie |
|
Western Region Lead AA |
C. Y. Hu |
|
North Central Region |
M. Jahn |
|
Northeastern Region |
E. Ashworth |
United States
Department of Agriculture
|
Agricultural Research
Service |
|
|
|
Technical Representative |
C. R. Brown |
|
|
National Program Staff |
P. K. Bretting G. C. Wisler |
|
|
Area Director, |
L. Chandler |
|
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Cooperative States Research Education & Extension Service |
A. M. Thro |
|
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Animal and Plant Health Inspection
Service |
M. D. Bandla |
|
|
NRSP-6 Project Leader |
J. B. Bamberg |
|
Agriculture
|
B. Bizimungu |
3. PROGRESS
AND PRINCIPAL ACCOMPLISHMENTS
A.
Introduction
of New Stocks
John Bamberg,
Alfonso del Rio and Charles Fernandez (US Potato Genebank) had a successful
collecting expedition to the
A
total of 99 accessions were assigned PI numbers in 2008, with 76 as true
seed: eight Bolivian accessions from
Spooner's 1993-4 collection trip, 68 accessions from the Polish seed rescue of
VIR stocks and 15 accessions collected in Arizona in 2008. Eight accessions were added to the tissue
culture collection: three varieties from
The NRSP-6 web page (http://www.ars-grin.gov/nr6) has been updated to include all new stocks and screening information. Clients who have ordered from NRSP-6 in the past four years are contacted three times per year informing them of new materials that are now available either as true seed, tubers, in vitro plantlets, or herbarium samples.
B. Preservation
and Evaluation of Stocks
In 2008, a total of 221 accessions were increased as botanical seed populations.
A total of 693 potato spindle tuber viroid (PSTVd) tests were performed on seed increase parents, seedlots and research materials. Germination tests were performed for 1497 accessions, ploidy determinations for 21, and tetrazolium seed viability tests for eight.
In cooperation with J. Palta (UW-Madison), it was found that low-cost calcium
applications in the highlands of
We continued screening for antioxidants and anti-tumor components with USDA cooperator R. Navarre (USDA-Prosser) and J.C. Miller, Jr. (TAMU). Some extremely high phenolic clones were found in S. phureja/stenotomum and extracts of S. jamesii were found to inhibit colon and prostate cancer. We documented and reported remarkable eight-year dormancy of S. jamesii tubers from stocks we collected from New Mexico.
C. Classification
Dr. Spooner et al. have published and are working on five different areas of potato research: 1) molecular markers for genebank studies, 2) cultivated potato origins, 3) relationships in wild tomatoes and potatoes, 4) the predictive power of taxonomy relative to disease resistance data, and 5) a linkage map for late blight resistance in wild potatoes.
D. Distribution
The volume and
types of stocks sent to various consignee categories are summarized in the
table below. NRSP-6 distributed 168
orders to clients in 30 states of the
|
Units of Germplasm Sent1 |
|
|||||||
|
Category |
S |
TF |
TC |
IVS |
DNA |
PL |
HER |
Total |
PIs |
|
Domestic |
1,854 |
565 |
1121 |
366 |
0 |
5 |
0 |
3,902 |
3,559 |
|
Foreign |
631 |
91 |
28 |
1,033 |
3 |
0 |
0 |
1,786 |
870 |
|
Total |
2,476 |
656 |
1149 |
1,399 |
3 |
5 |
0 |
5,688 |
4,429 |
1 Types of stocks sent/(number of seeds, tubers or plantlets per standard shipping
unit): S= True Seeds/(50), TF= Tuber Families/(10),
TC=Tuber Clones/(3),
IVS=In Vitro Stocks/(1), DNA=DNA samples/(1), PL=Plants in plugs/(1), Her= Herbarium/(1).
4. IMPACT STATEMENT
Potato is the number one US and world vegetable in terms of production, value, and consumption. Considering its high satiety index and palatability, and its balanced protein, wide adaptability, and high productivity, it will play an increasingly important role in providing food security in developing countries and delivering new health-promoting nutrients to the diets of developed countries. Such food and health benefits carry with them a great economic impact, even in areas where potatoes are not grown. For example, if people in the US consumed adequate potassium, an estimated 100K lives and $12B in annual healthcare costs would be saved. Potato, already a high-potassium food, is well positioned to make a substantial contribution through genetic improvement. NRSP-6, as the world’s most diverse and available source of new genes and germplasm information, is best positioned to support such contributions.
Beyond providing stocks, NRSP-6 staff members are involved in discovering and developing associated germplasm tools and information. Among these are self compatibility, gibberellin, and 2n gamete mutants; cut-stem pollination, hormone pre-treatment of seeds for better germination, haploid- extracting pollinators, and 2n gamete breeding technique. Yukon Gold, one of the most popular and name-recognized tablestock cultivars, has S. phureja 195198, an exotic cultivated species from NRSP-6 as a grandparent, and was bred using the 2n gamete technique.
Evaluation for a wide variety of useful traits has also been designed, contracted and documented by staff. Such work is the foundation for deploying exotic genes in new cultivars. One recent example is the release of cultivar PA99N82-4 bred with the Mexican wild species S. bulbocastanum from NRSP-6. It has high resistance to nematodes that can only be controlled by fumigation at an estimated cost of $20M per year, not counting the “cost” in risks to human and environmental health posed by use of toxic chemicals.
The genebank goal is maximum diversity. But because funds for collecting, preserving, distributing and evaluating are limited, reaching that goal depends on maximizing efficiency through quality control and technology R&D. Thus, we collaborate with other world genebanks to study the partitioning and vulnerability of diversity in our collections. Examples of impact of this area are the intergenebank potato database, identification of more diversity-intense sites for future collecting, and confirming that the rare alleles within some populations within certain species are not explained by introgression of alleles common in another sympatric species.
One way the overall impact of
these contributions can be measured is by the occurrence of NRSP-6 germplasm in
the pedigrees of new, improved potato cultivars. About 70% of all potatoes grown in the
Another gauge of impact is in the numerous publications in 2008 providing information that pushes potato science forward. Section 6 lists 37 papers, 85 abstracts, and 4 theses which report the results of studies associated with NRSP-6 Solanum stocks this year.
The impact of the genebank is expected to increase in the future for several reasons. 1) Mutants discovered and characterized by staff will be increasingly valuable as research models. 2) Intragenic transformation of potato has now been demonstrated and identified as a kind of GMO much more accepted by the consumer, so useful exotic potato genes will be increasingly valuable as the technology to easily insert them into existing cultivars improves. 3) Potato is rapidly expanding in large new growing regions, so the need for genetic resources for breeding in new environments and for new tastes will surge. 4) Loss of wild habitats and other limits on collecting will make it even more important to understand how to efficiently keep what we already have—thus, enhancing the importance of in-house R&D on the partitioning and vulnerability of diversity. 5) The revolution in electronic information exchange gives NRSP-6 an opportunity to provide more complete and timely germplasm data, advice, and stocks, and detect and develop opportunities for new traits and germplasm applications. 6) Potato genetic resources will be increasingly mined for nutritional traits that reduce healthcare costs and suffering as evaluation and breeding technology advances.
5. WORK PLANNED FOR 2009
Fast and accurate delivery of high quality germplasm and information will continue to be the general objective of NRSP-6. We also aim to raise awareness of the germplasm resource through an advertising/outreach program, and by conducting and publishing research that demonstrates new ways the germplasm can be useful for potato improvement. It will be a goal to perform 250 successful seed increases in the upcoming year.
Evaluation experiments will continue on Solanum species for these and other traits: antioxidants, tuber acidity, tuber potassium, frost hardiness, tuber calcium, hormone mutants and anti-cancer compounds.
APIC Intergenebank projects, such as researching the status and dynamics of genetic diversity using DNA markers, will strengthen ties with sister genebanks around the world. Specifically, ongoing joint studies on tuber calcium, frost hardiness, and impact of agrichemicals on reproduction of wild potato populations will continue.
Our
plan is to move toward consolidation of R&D into a single major project
that encompasses most of our priority goals:
Intergenebank cooperation, detection of how
diversity is partitioned and how it is vulnerable, evaluation for useful
traits, and emphasis on evaluation for consumer-oriented traits. These activities will center on S. microdontum,
outstanding as a species with extreme and variable expression for many
potentially useful traits (pH, potassium, calcium, late blight and soft rot
resistance, reproductive mutants, high protein and antioxidants), while being
relatively easy to grow and introgress into S. tuberosum. This is the “MMP” = microdontum multifaceted project. A
multifaceted approach should be more efficient, since multiple evaluations can
be done on tubers from one growout, and interactions
between traits can be detected.
We intend to
conduct a collecting expedition for native wild potatoes in the
6. PUBLICATIONS
ISSUED DURING THE YEAR 2008
Publications
issued by NRSP-6 Personnel
Alvarez, N.M.B., I.E. Peralta, A. Salas, and D.M. Spooner.
2008. A morphological study of species boundaries of the wild potato Solanum brevicaule
complex: replicated field trials in
Belmar-Diaz, C., H. Lozoya-Saldana, M.
Salgado, and J. Bamberg. 2008. Phytophthora
infestans: races and genotypes in
Fajardo, D., R. Castillo, A Salas, and D.M. Spooner. 2008. A morphometric study of species boundaries of the wild potato Solanum series Conicibaccata: a replicated field trial in Andean Peru. Syst Bot 33:183-192.
Hale, Anna L., Lavanya Reddivari, M. Ndambe Nzaramba, John B. Bamberg,
and
Jansky, S.H., R. Simon and D.M. Spooner. 2008. A test of taxonomic predictivity: resistance to early blight in wild relatives of cultivated potato. Phytopath 98:680-687.
Jimenez, J.P., A. Brenes, A. Salas, D. Fajardo, and D.M. Spooner. 2008. The use and limits of AFLP data in the taxonomy of polyploid wild potato species in Solanum series Conicibaccata. Conserv Genet 9:381-387.
Nzaramba, M.N.,
L. Reddivari, J.B. Bamberg, and J.C. Miller Jr. 2008.
Phenolic
and glycoalkaloid levels of S. jamesii
accessions and their anti-proliferative effect on human prostate and colon
cancer cells in vitro. 92nd Annual Meeting of The PAA,
Palta, J.P., J.B. Bamberg and S.E. Vega. 2008. Moving frost hardy genes from wild to cultivated potatoes. Use of precise screening tools to make real progress. Am J Potato Res 85:23. (Abstract)
Palta, J.P., J.B. Bamberg and S.E. Vega. 2008. Freezing tolerance of cultivated potatoes: Moving frost hardy genes from wild potatoes and making real progress using precise screening tools. ASHS 2008 Conference, Orlando, Florida. HortSci 43:1108. (Abstract)
Palta, J.P.,
J.B. Bamberg, S.E. Vega, F.M. Navarro, and B. Bowen. 2008. Genetic
improvement of potato for tuber calcium uptake. Proceedings of Annual
Palta, J.P., R. Gomez, A.H. del Rio, W. Roca, J.B. Bamberg, A. Salas, and M. Bonierbale. 2008. Supplemental calcium nutrition may have the potential of improving tuber yield of native potatoes in the Peruvian highlands. Am J Potato Res 85:23. (Abstract)
Palta, J.P., F.M. Navarro, J.B. Bamberg, S.E. Vega, and B. Bowen. 2008. The Calcium Solution: Developing potato cultivars with enhanced tuber storage and internal quality by genetic improvement of tuber calcium accumulation ability. The Badger Common ’Tater 60(11):14-16.
Pendinen G., T. Gavrilenko, J. Jiang, and D.M. Spooner. 2008. Allopolyploid speciation of the Mexican tetraploid potato species Solanum stoloniferum and S. hjertingii revealed by genomic in situ hybridization. Genome 51:714-720.
Peralta, I.E., D.M. Spooner and S. Knapp. 2008. The taxonomy of tomatoes: a revision of wild tomatoes (Solanum section Lycopersicon) and their outgroup relatives in sections Juglandifolium and Lycopersicoides. Syst Bot Monogr 84:1-186+3 plates.
Spooner, D.M., D. Fajardo and A. Salas. 2008. Revision of the Solanum medians complex (Solanum sect. Petota). Syst Bot:33:579-588.
Spooner, D.M., F. Rodriquez, Z. Polgar, H.E. Ballard Jr., and S.H. Jansky. 2008. Genomic origins of potato polyploids: GBSSI gene sequencing data. The Pl Genome, a Suppl. to Crop Sci. 48(S1):S27-S36.
Journal Articles and Abstracts Reporting Research with NRSP-6 Stocks
Almasia, N.I., A.A. Bazzini, H.E. Hopp, and C. Vazquez-Rovere. 2008. Over expression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants. Mol Pl Path 9(3):329-338. (Abstract)
Arvin, M.J. and D.J. Donnelly. 2008. Screening potato cultivars and wild species to abiotic stresses using an electrolyte leakage bioassay. J Ag Sci Tech 10(1):33-42. (Abstract)
Aversano, R., M.R. Ercolano, L. Frusciante, L. Monti, J.M. Bradeen, G. Cristinzio, A. Zoina, N. Greco, S. Vitale, and D. Carputo. 2007. Resistance traits and AFLP characterization of diploid primitive tuber-bearing potatoes. Genet Res Cr Evol 54(8):1797-1806. (Abstract)
Ballou, S.M., K.Y. Yun, C. Cheng, and B.G. de los Reyes. 2007. Cold sensitivity gradient in tuber-bearing Solanum based on physiological and transcript profiles. Crop Sci 47(5):2027-2035. (Abstract)
Bhaskar, P.B., J.A. Raasch, L.C.
Kramer, P. Neumann, S.M. Wielgus, S. Austin-Phillips,
and J.M. Jiang. 2008. Sgt1, but not Rar1, is essential for the RB-mediated
broad-spectrum resistance to potato late blight.
Bidani, A., O. Nouri-Ellouz, L. Lakhoua, D. Sihachakr, C. Cheniclet, A. Mahjoub, N. Drira, and R. Gargouri-Bouzid. 2007. Interspecific potato somatic hybrids between Solanum berthaultii and Solanum tuberosum L. showed recombinant plastome and improved tolerance to salinity. Pl Cell, Tissue & Organ Culture 91(3):179-189. (Abstract)
Bradeen, J.M., M. Iorizzo, H.
Mann, L. Gao, E.A. Quirin,
R. Aversano, and D. Carputo.
2008. Comparative structural genomics of 1EBN potato: DArT
markers for improved access to resistance genes. 92nd Annual Meeting
of The PAA,
Brown, C.R. 2008. Breeding for phytonutrient enhancement of potato. Am J Potato Res 85:298-307.
Brown, C.R., D. Culley, M. Bonierbale, and W. Amoros. 2007. Anthocyanin, Carotenoid content, and antioxidant values in native South American potato cultivars. HortSci 42(7):1733-1736. (Abstract)
Brown, C.R., M.
Moore, M. Pavek, D. Hane,
R. Novy, J.C. Miller Jr., S.L. Love, and S. James. 2008. Genetic variability in
mineral content of potato tubers. 92nd Annual Meeting of The PAA,
Burkhart, C.R., B.J. Christ, K.G. Haynes, and B.T. Vinyard. 2008. Little genetic and no additive
genetic variance for resistance to common scab in a Solanum
phureja-S. stenotomum
population. 92nd Annual Meeting of The PAA,
Camadro, E.L., S.K. Saffarano, J.C. Espinillo, M. Castro, and P.W. Simon. 2008. Cytological mechanisms of 2n pollen formation in the wild potato Solanum okadae and pollen-pistil relations with the cultivated potato, Solanum tuberosum. Genet Res Crop Evol 55(3):471-477. (Abstract)
Campbell, B.A., J. Hallengren and D.J. Hannapel. 2008. Accumulation of BEL1-like transcripts in solanaceous species. Planta 228:897-906.
Cernak, I., J. Taller, I. Wolf, E. Feher, G. Babinszky, Z. Alfoldi, G. Csanadi, and Z. Polgar. 2008. Analysis of the applicability of molecular markers linked to the PVY extreme resistance gene Rysto, and the identification of new markers. Acta Bio Hungarica 59(2):195-203. (Abstract)
Chatterjee, M., A.K. Banerjee and D.J. Hannapel. 2007. A BELL1-like gene of potato is light activated and would inducible. Pl Physio 145(4):1435-1443. (Abstract)
Cheng, ShanHan, Xie CongHua,
Lin ShiSen et al. 2006. Germplasm
enhancement and breeding to resist low temperature sweetening in potato.
Coleman, W.K. 2008. Evaluation of wild Solanum
species for drought resistance: 1. Solanum gandarillasii Cardenas.
Environ Exp Bot 62(3):221-230. (Abstract)
D’hoop, B.B. 2008. Association mapping in tetraploid potato using SSRs and AFLPS. In: 17th Triennial Conference of the EAPR, Abstracts of papers and posters, Brasov, Romania, July 6-10, 2008, p. 376. (Abstract)
D’hoop, B.B.,
M.J. Paulo, K. Kowitwanich, R.G.F. Visser, H.J. van Eck, and F.A. van Eeuwijk.
2008. Association mapping in tetraploid potato using
SSRs and AFLPs. In: Molecular mapping and marker assisted selection in plants, Programme and abstracts,
Douches, D., J. Coombs, J. Estelle, D. Berry, K. Zarka, W. Kirk, and R. Schafer. 2008. Update on late
blight resistance breeding in
Drobyazina, P.
and E. Khavkin. 2008. Two CONSTANS-LIKE1
genes in long- and short-day Solanum plants. XVI FESPB Congress, Tampere, Finland, 2008.
Drobyazina, P.E. and E.E. Khavkin. 2008. Two CONSTANS-LIKE genes in Solanum plants. Control of flowering time and application for plant breeding. Science meeting, Salzau, German, September 2008, p 35. (Abstract)
Fry, W. 2008. Phytophthora infestans: the plant (and R gene) destroyer. Mol Pl Path 9(3):385-402. (Abstract)
Ginzberg, I., J. Tokuhisa and R.E. Veilleux. 2008. Potato steroidal glycoalkaloids: Biosynthesis and genetic manipulation. Potato Res 52:0014-3065 (Print) 1871-4528 (Online).
Gokce, A., M.E. Whalon, H. Cam, Y. Yanar, I. Demirtas, and N. Goren. 2007. Contact and residual
toxicities of 30 plant extracts to Colorado potato beetle larvae. Archives
Govers, F. and H.J.G. Meijer. 2007. Phytophthora genomics: new opportunities and challenges. Gewasbescherming 38(5):265-271. (Abstract)
Greco, N., A. Brandonisio, and P. de Cosmis. 2007. Pathotypes and heterogeneity of Italian populations of Globodera rostochiensis and G. pallida. Nematol Mediterranea 35(2):137-142. (Abstract)
Halterman, D.,
Z. Liu, Y. Chen, and S. Stephenson. 2008. Molecular characterization of
potato disease resistance genes. 92nd Annual Meeting of The PAA,
Halterman, D.A., L.C. Kramer, S. Wielgus, and J.M. Jiang. 2008. Performance of transgenic potato containing the late blight resistance gene RB. Pl Disease 92(3):339-343. (Abstract)
Hannapel, D.J.,
M. Chatterjee, Y.Y. Yu, H. Chen, and A.K. Banerjee. 2008. A light-activated signal for tuberization. 92nd Annual Meeting of The PAA,
Haynes, Kathleen G. 2008. Heritability of chip color and specific gravity in a long-day adapted Solanum phureja-S. stenotomum population. Am J Potato Res 85:361-366.
Haynes, K.G., B.J. Christ and B.T. Vinyard. 2008. Determining the importance of combining ability for late blight resistance in early generations of potato breeding when susceptible clones are discarded. Am J Potato Res 85:445-454.
Heldak, J., M. Bezo, V. Stefunova, and A. Gallikova. 2007. Selection of DNA markers for
detection of extreme resistance to potato virus Y in tetraploid
potato (Solanum tuberosum L.) F1 progenies.
Hermanova, V.,
J. Barta and V. Curn.
2007. Wild potato species: Characterization and biological potential for potato
breeding.
Horgan, F.G., D.T. Quiring, A. Lagnaoui, and Y. Pelletier. 2007. Variable responses of tuber moth to the leaf trichomes of wild potatoes. Ent Exp Appl 125(1):1-12. (Abstract)
Horgan, F.G., D.T. Quiring, A. Lagnaoui, A.R. Salas, and Y. Pelletier. 2007. Periderm- and cortex-based resistance to tuber-feeding Phthorimaea operculella in two wild potato species. Ent Exp Appl 125(3):249-258. (Abstract)
Houser, A.J. and R. Davidson. 2008. Development of a greenhouse
assay to evaluate potato germplasm for susceptibility to powdery scab. 92nd
Annual Meeting of The PAA,
Jacobs, M.M.J., R.G. van den Berg, V.G.A.A. Vleeshouwers, M. Visser, R. Mank, M. Sengers, R. Hoekstra, and B. Vosman. 2008. AFLP analysis reveals a lack of phylogenetic structure with Solanum section Petota. BMC Evol Bio 88(145). (Abstract)
Jansky, S.H., A.J. Hamernik and J. Bae. 2008. The use of Solanum
verrucosum to access 2x, 1EBN germplasm. 92nd
Annual Meeting of The PAA,
Jolivet, K., E. Grenier, J.P. Bouchet, M. Esquibet, M.C. Kerlan, B. Caromel, D. Mugniery, and V. Lefebvre. 2007. Identification of plant genes regulated in resistant potato Solanum sparsipilum during the early stages of infection by Globodera pallida. Genome 50(4):422-427. (Abstract)
Kielbowicz-Matuk, Agnieszka, Pascal Rey and Tadeusz Rorat. 2008. The organ-dependent abundance of a Solanum lipid transfer protein is up-regulated upon osmotic constraints and associated with cold acclimation ability. J Exp Bot, 4/25/2008, pp 1-13.
Lang, ZhiHong, Zhou Peng, Yu JingJuan, Ao GuangMing, and Zhao Qian. 2008. Functional characterization of the pollen-specific SBgLR promoter from potato (Solanum tuberosum L.). Planta 227(2):387-396. (Abstract)
Le Roux, V., E.Campan, F. Dubois, C. Vincent, and P Giordanengo. 2007. Screening for resistance against Myzus persicae and Macrosiphum euphorbiae among wild Solanum. Ann. Appl. Biol. 151:83-88.
Le Roux, V., S. Dugravot, E.Campan, F. Dubois, C. Vincent, and P Giordanengo. 2008. Wild Solanum Resistance to Aphids: Antixenosis or Antibiosis? J. of Econ. Entomol. 101 (2): 584-591
Liu, XiCai. 2006. Study and utilization of potato germplasm
resources.
Love, S.L. and J.J. Pavek. 2008. Positioning the potato as a primary food source of vitamin C. Am J Potato Res 85:277-285.
Luthra, S.K., J. Gopal, V. Kumar, B.P. Singh, and S.K. Pandey. 2008. Evaluation of potato germplasm for frost tolerance. Indian J Hort 65(3):344-346. (Abstract)
McCue, K.F., D.R. Rockhold, P.V. Allen,
and W.R. Belknap. 2008. Useful promoters and terminators from Solanum bulbocastanum
for use in potatoes and other crops. 92nd Annual Meeting of The PAA,
Morante, M.C.,
I. Tolin Tordoya and N. Montealegre Villanueva. 2007. Resistance to Phytophthora infestans
Mont de Bary of phureja
potato (Solanum phureja
Juzepczuk et Bukasov) and populations of wild species of potato,
Morris, W.L., H.A. Ross, L.J.M. Ducreux, J.E. Bradshaw, G.J. Bryan, and M.A. Taylor. 2007. Umami compounds are a determinant of the flavor of potato (Solanum tuberosum L.). J Ag Food Chem 55(23):9627-9633. (Abstract)
Mosley, A.R.,
S. Yilma, D.C. Hane, S.R.
James, K.A. Rykbost, C.C. Shock, S.L. Love, D.L. Corsini, J.J. Pavek, R.E.
Thornton, B.A. Charlton, E.P. Eldredge, R.G. Novy,
M.J. Pavek, N.R. Knowles, J.L. Whitworth, C.R. Brown,
J.C. Stark, and M.I. Vales. 2008.
Mugniery, D., O. Plantard, S. Fournet, E. Grenier, B. Caromel, M.C. Kerlan, D. Picard, and D. Ellisseche. 2007. Evaluation of effectiveness and durability of resistance to Globodera pallida from Solanum vernei, S. spegazzinii and S. sparsipilum. Nematol Mediterranea 35(2):143-153. (Abstract)
Nichol, N.L., K. Zarka, J. Coombs, and
D.S. Douches. 2008. CBF1 transgenic potatoes studied under
drought conditions. 92nd Annual Meeting of The
PAA,
Novy, R.G., K.B. Kelley and J.L. Whitworth. 2008. Introgression of virus resistances from Solanum etuberosum into cultivated potato and the identification of molecular markers linked to it potato leafroll virus (PLRV) resistance. The 17th Triennial Conf. of the European Assoc. for Potato Research, Brasov, Romania. 7/8/08. Abstracts of Papers and Posters, pp. 70-71. (Abstract)
Novy, R.G., J.L. Whitworth, J.C. Stark, S.L. Love, D.L. Corsini, J.J. Pavek, M.I. Vales, S.R. James, D.C. Hane, C.C. Shock, B.A. Charlton, C.R. Brown, N.R. Knowles, M.J. Pavek, T.L. Brandt, and N. Olsen. 2008. Premier Russet: A dual-purpose, potato cultivar with significant resistance to low temperature sweetening during long-term storage. Am J Potato Res 85:198-209.
Pelletier, Y. and D. De Koeyer. 2008. The use of Solanum oplocense as a source of resistance to the Colorado potato beetle, Leptinotarsa decemlineata (Say.). Symposium “Insects of Solanaceous crop” at the International Congress of Entomology, July 6-12, 2008, Durban, South Africa.
Pelletier, Y. and D. De Koeyer. 2008. Solanum oplocense as a source of resistance to the Colorado potato beetle and improved processing quality. XXIIIrd Association Latino Americano de la Papa Annual, Mar del Plata, Argentina, Nov. 30th to Dec. 5th. (Poster)
Peloquin, S.J., L.S. Boiteux, P.W. Simon, and S.H. Jansky. 2008. A Chromosome-specific estimate of transmission of heterozygosity by 2n gametes in potato. J Heredity 99(@):177-181. (Abstract)
Pennycooke, J.C., H.M. Cheng, S.M. Roberts, Q.F. Yang, S.Y. Rhee, and E.J. Stockinger. 2008. The low temperature-responsive, Solanum CBF1 genes maintain high identity in their upstream regions in a genomic environment undergoing gene duplications, deletions, and rearrangements. Pl Mol Bio 67(5):483-497. (Abstract)
Pino, M.T., J.S. Skinner, Z. Jeknic, P.M. Hayes, A.H. Soeldner, M.F. Thomashow, and T.H.H. Chen. 2008. Ectopic AtCBF1 over-expression enhances freezing tolerance and induces cold acclimation-associated physiological modifications in potato. Pl Cell Environ 31(4):393-406. (Abstract)
Polkowska-Kowalczyk, L., B. Wielgat and U. Maciejewska. 2007. Changes in the antioxidant status in leaves of Solanum species in response to elicitor from Phytophthora infestans. J Pl Physio 164(10):1268-1277. (Abstract)
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7.
APPROVED
__________________________________________ __________
D. S. Douches, Chairman, Technical Committee Date
__________________________________________ __________
C. Y. Hu , Lead Administrative Advisor Date