Minutes – 2003 Tomato Crop Germplasm Committee
April 28, 2003 Park City, Utah

Members Present:

John Stommel (Chair)
Martha Mutschler (Vice Chair)
Mark Barineau
Roger Chetelat
David Francis*
Randy Gardner*
Bob Heisey
Rich Ozminkowski
Susan Peters*
Larry Robertson
Jay Scott
Ira Stein
Al Stoner
Cathy Thome*
Ray Volin*

* New members approved at this meeting

Additional Participants:
Fred Angel, Fernando Liaoza, John Prendergast, Felix, Jeff Ziscke, Joanne Labate, Remo Ludergnani, Sylvaine

Meeting was called to order at 7:50 pm and the minutes from the 2001 meeting in Guatemala were approved.

USDA, ARS, Geneva curator report: L. Robertson
- A final L. esculentum descriptor list has been submitted to GRIN.
- 401 accession fruit or leaf images have been uploaded to GRIN. R. Heisey noted discrepancies between photos and descriptors at GRIN. Larry indicated that the photos are correct and descriptor listings warrant editing.
- Seed increases of 61 unique NCGRP (formerly NSSL) accessions have been initiated and PI numbers requested. 140 remaining accessions unique to NCGRP will be increased in 2004 and be made available for distribution.
-An accession series, T001 – T171 originating from Oklahoma and dated through 1969, has been received. No passport information is available.
- Core Collection: 140 accessions for the L. esculentum core have been increased for distribution. Viable seed of three of the originally selected lines is unavailable. A. Stoner described the L. esculentum core as a collection of cultivated tomato germplasm representative of open-pollinated cultivars from public and private breeding programs that broadly represents the diversity within cultivated L. esculentum and the larger Geneva collection. It may serve as a historical record of early breeding programs. Much of this material contributed to the development of modern day cultivars. It is recommended that additional material be added or deleted as further genetic characterization of accessions occurs. The core serves as an entry point for identification or characterization of attributes in the larger collection. D. Francis commented that additional cores might be assembled to reflect, for example, geographic distribution of L. esculentum. He suggested that European material is under represented. R. Heisey suggested considering newer material, such as ‘Piedmont’. R. Heisey volunteered to contact F. Angel, D. Francis, R. Gardner, J. Prendergast, and J. Scott to assemble a supplementary list of material for consideration in the core. J. Stommel cautioned that new varieties are typically protected and hence are inappropriate for the collection. The committee agreed that the new L. esculentum core list was an excellent start and that the core collection should be updated as new information and uses develop.
- The tomatillo collection is curated at Geneva. The CGC discussed the merit of adding tomatillo to the Tomato CGCs responsibilities. Approximately 70% of the requests for tomatillo germplasm are domestic. International distributions have been limited due in part to permit requirements. The CGC does not believe that adequate expertise exists on the committee to represent tomatillo and declined assuming responsibilities for the collection until sufficient need arises.
- The committee briefly discussed usage of Solanum versus Lycopersicon taxonomic nomenclature and agreed to continue use of Lycopersicon. J. Stommel noted the opportunity to invite D. Spooner, a proponent of adopting Solanum, to present his findings to the Tomato Breeders Roundtable.

Tomato Genetics Resource Center report: R. Chetelat
- A complete TGRC report was distributed prior to the CGC meeting.
- Roger noted that Center’s lab facilities have been upgraded. Greenhouse and other costs have been rising and are exacerbated by current California budgetary problems. Notable new acquisitions included a set of 94 L. pimpinellifolium backcross recombinant inbred lines and multigenic combinations submitted by D. Zamir.
- Distributions remain high and are indicative of the importance of the collection. An increasing number of countries now require phytosanitary certificates and/or import permits. A bar coding system is needed to facilitate inventory and shipment record keeping.
- An updated stock list will be published in the 2003 Tomato Genetics Cooperative Report.

GRIN/TGRC databases: A. Stoner
- Discussions to better coordinate GRIN and TGRC databases were initiated in 2002. Current efforts are focused on establishing better links between the databases. Elements unique to the respective databases hinder creation of a single database. XML-based links will limit the need for cumbersome manual updates of GRIN data to TGRC and facilitate simultaneous searches of the two databases.

New PGRU initiative: J. Labate
- J. Labate presented plans to identify and utilize SNPs for characterization of the tomato collection at Geneva. The project will initially utilize 30 loci and focus on the L. esculentum core collection and 458 PI species accessions.
- D. Francis expressed concern that the USDA project initiated by Joanne was competitive with existing state research programs and that the initial effort was focused on a limited number of genes.
- An analogous effort using SSRs was conducted by R. Chetelat to characterize L. chilense accessions.

L. esculentum collection management:
Approximately 1200 potential duplicate accessions are present in the USDA collection at Geneva. This is a recurring discussion for the CGC. A. Stoner has agreed to review the passport information in an effort to make initial recommendations to eliminate duplicate accessions. L. Robertson has agreed to supply an updated duplicates list to the CGC and J. Stommel and A. Stoner will assign grow-out responsibilities for relevant accessions to committee volunteers. Criteria for accession retention will be contingent upon passport information, prior accession usage, and grow-out findings. Volunteers to grow-out: Scott, Stommel, Chetelat, Gardner, Barineau, Francis, Stein, Thome, Peters, Ludergnani, Angel, Ozminkowski

Germplasm evaluation and exploration proposals: J. Stommel
- USDA had not yet finalized funding decisions for CGC germplasm evaluation proposals. The CGC forwarded three proposals for funding consideration: Evaluation of the L. hirsutum core collection for resistance to bacterial spot and bacterial canker; Dave Francis, Ohio State University (CGC rank: 48 points); Screening the Lycopersicon peruvianum germplasm collection for resistance to isolates of tomato spotted wilt virus that overcome the tomato Sw-5 TSWV resistance gene; Mikel Stevens, Brigham Young University (CGC rank: 46 points); Evaluation of tomato germplasm for resistance to Fusarium foot rot; Mike Davis, University of California (CGC rank: 28 points). (Postscript to Park City meeting: USDA awarded $16,000 in funds to support the Mikel Stevens proposal. Funds for individual proposals submitted for FY2003 funding were capped at $16,000-$18,000 and the D. Francis proposal was deemed not feasible at the reduced funding level. Funding for FY2004 proposals will also be capped at $16,000-$18,000).
- Improved protocols are often needed to facilitate screening for a particular attribute. Proposals may focus on methods development. J. Stommel noted the opportunity to evaluate introgression lines that are available in NPGS collections.
- A. Stoner noted that increased funding for tomato germplasm was unlikely unless funding for the larger USDA germplasm evaluation program was increased.
- A final report was submitted by Mike Davis detailing results from his 2002-funded proposal for evaluation of tomato germplasm resistance to Fusarium foot rot. The results will be uploaded by TGRC staff to the TGRC database.
- Funds are again available for funding tomato exploration proposals. The proposal deadline is July 3, 2003.

CGC business / membership:
- Many of the CGCs have been lax in updating their reports. The Tomato CGC Report was last updated in 1996. J. Stommel will coordinate revision and submit an updated report to A. Stoner.
- J. Stommel rotates off as chair and M. Mutschler assumes chair duties in 2004. D. Francis elected vice-chair for 2004. Both 3-year terms.
- Randy Gardner, Susan Peters, Cathy Thome, Ray Volin, and David Francis have been serving as ad hoc members since the 2001 Antigua meeting. The CGC formally approved their appointments at the Park City meeting.
- Additional members are needed to represent West coast processing. Suggested individuals were T. Beck-Bunn, P. Himmel, G. Pape, and D. Long. New members will be confirmed and ratified via e-mail to CGC members prior to the next meeting.

Meeting adjourned 10:15 pm.