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Christina Walters (chrisv@lamar.colostate.edu)
Biophysical and
physiological aspects of seed development and quality
Education:
B.S. Plant Sciences, Cornell University, 1981
Ph.D. Botany, Cornell University, 1986
Research Interests:
Plant embryo development and quality
Desiccation tolerance and dry biological systems
Biophysical aspects of water properties
Cryobiology
Conservation of genetic resources
Other Activities:
Editorial Board, Seed Science Research (since 1994), CryoLetters
(1995-2002).
Elected Chair CSSA C8 Division (plant genetic resources) (2003-2005)
Elected Member-at-large AAAS (Agriculture Division) (2004-2006)
Science Advisory Committee, Center for Plant Conservation (1999- present)
Selected Publications:
Walters, C., L.J. Wheeler, and J. Grotenhuis. 2005. Longevity of seeds
stored in a genebank: species characteristics. Seed Science Research
15:1-20.
Walters, C., L.J. Wheeler, and P.C. Stanwood. 2004. Longevity of cryogenically-stored
seeds. Cryobiology 48:229-244.
Walters, C. 2004. Temperature-dependency of molecular mobility in preserved
seeds. Biophysical Journal 86: 1253-1258.
Wesley-Smith, J., C. Walters, P. Berjak, and N.W. Pammenter. 2004.
The influence of cytoplasmic viscosity, cooling and warming rate upon
survival of embryonic axes of Poncirus trifolata (L.). CryoLetters 25:129-138.
Walters, C, JM Farrant, NW Pammenter and P Berjak. 2002. Desiccation
Stress and Damage. In M. Black and H.W. Pritchard (eds) Desiccation
and Plant Survival. CABI Publishing, Wallingford, UK pp. 263-291.
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Leigh E. Towill
(ltowill@lamar.colostate.edu)
Cryobiology of Vegetatively
Propagated Germplasm
Education:
B.S. Botany, University of Wisconsin-Milwaukee, 1966
M.S. Botany, University of Michigan, 1968
Ph.D. Botany, Plant Physiology, University of Michigan, 1972
Research Interests:
Plant stress physiology
Cryobiology of plant tissues
Cryopreservation of vegetatively propagated germplasm
Mechanisms of desiccation and low temperature injury in vegetative propagules
Development of viability assays for plant tissues
In vitro propagation
Other Activities:
Editorial Board, CryoLetters (2004-present), Journal of the American Society
for Horticultural Science (1998-2002)
Selected Publications:
Towill, L.E., P.L. Forsline, C. Walters, J. Waddell, and J. Laufmann.
2004 Cryopreservation of Malus germplasm using a winter vegetative
bud method: results from 1915 accessions. CryoLetters 25:323-334.
Towill, L.E. and M. Widrlechner. 2004. Cryopreservation of Salix
species using sections from winter vegetative scions. CryoLetters 25:71-80.
Towill, L.E. and R. Bonnart. 2003. Cracking in a vitrification solution
during cooling or warming does not affect growth of cryopreserved mint
shoot tips. CryoLetters 24:341-346.
Towill, L.E. and Y.P.S. Bajaj (eds.). 2002. Cryopreservation of Plant
Germplasm II. Biotechnology in Agriculture and Forestry Series Vol 50.,
396 pp. Springer, London.
Towill, L.E. 2002. Cryopreservation of plant germplasm: Introduction
and some observations. pp. 3-21. In L.E. Towill and Y.P.S. Bajaj (eds.)
Cryopreservation of Plant Germplasm II. Biotechnology in Agriculture
and Forestry Series vol 50. Springer, London.
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Gayle M. Volk (gvolk@lamar.colostate.edu)
Cellular and molecular aspects
of plant cryopreservation
Education:
B.S. Biochemistry, Bioagricultural Sciences, Colorado State Univ.
1991
M.S. Horticulture, Purdue University, 1993
Ph.D. Plant Physiology, Cornell University, 1998
Research Interests:
Cryopreservation of vegetatively propagated materials
Biophysical and ultrastructural changes in meristem cells during cryopreservation
Physiological and molecular effects during cryopreservation and recovery
Plant cell ultrastructure and microscopy
Genetic diversity and erosion in wild-collected germplasm of fruit crops
Selected Publications:
Volk, G.M., C.M. Richards, A.A. Reilley, A.D. Henk, P.L. Forsline,
and H.S. Aldwinckle. 2005. Ex situ conservation of vegetatively-propagated
species: Development of a seed-base core collection for Malus sieversii.
Journal of the American Society for Horticultural Science (in press,
March issue).
Volk, G.M., J.L. Harris, and K.E. Rotindo. 2005. Damaging effects of
cryoprotectant solution components in mint shoot tips. CryoLetters (submitted).
Volk, G.M., A.D. Henk, and C.M. Richards. 2004. Genetic diversity among
US garlic clones as detected using AFLP methods. Journal of the American
Society for Horticultural Science 129:559-569.
Volk, G.M., N. Maness, and K. Rotindo. 2004. Cryopreservation of garlic
(Allium sativum L.) using plant vitrification solution 2. CryoLetters
25:219-226.
Volk, G.M., and C. Walters. 2003. Preservation of genetic resources
in the National Plant Germplasm clonal collections. Plant Breeding Reviews
23: 291-344.
Galactinol synthase gene information:
GAS c DNA alignments.txt
Non-truncated GAS alignments.txt
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Christopher M Richards
(chris.richards@colostate.edu)
Population genetics
and genomics
Education:
B.A. Lewis and Clark College, Biology/English, 1984
M.S. Stanford University, Biological Sciences, 1986
Ph.D. Duke University, Genetics/ Botany, 1997
Research Interests:
Ecological and metapopulation genetics
Molecular evolutionary genetics
Inference of population structure and history from genetic data
Conservation genetics of ex situ collections
Selected Publications:
Volk, G., A. Henk, and C.M. Richards. 2004. Genetic diversity among
US garlic clones as detected using AFLP methods. Journal of the American
Society for Horticultural Science 129:559-569.
Richards, C.M., A.A. Reilley, D.H. Touchell, M.F. Antolin and C. Walters.
2004. Microsatellite primers for Texas wildrice (Zizania texana),
and a preliminary test of the impact of cryogenic storage on allele frequency
at these loci. Conservation Genetics 5:853-859.
Richards, C.M. 2004. Molecular technologies for the management and
use of gene bank collections. In (M.C. De Vicente, ed.) Issues
in Genetic Resources 11:13-18. IPGRI, Rome, Italy.
Richards, C. M., D.E. McCauley and S.N. Emery. 2003. Genetic and demographic
dynamics in colonizing populations of Silene alba. Heredity
90:181-186.
Richards, C. M. 2000. Inbreeding depression and genetic rescue in a
plant metapopulation. American Naturalist 155: 383-394.
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Jennifer Crane (jcrane@lamar.colostate.edu)
Biophysical and physiological
aspects of seed development and quality
Education:
B.A. Carleton College, Biological Sciences, 1979
M.S. Colorado State University, Horticultural Science, 1984
Research Interests:
Identify physiology of seeds for undomesticated plant species
Develop long-term storage protocols for intermediate and recalcitrant
seeds
Determine effects of age, maturity, and growth conditions on seed survival
during cryo-storage.
Selected Publications:
Vertucci, C.W., J. Crane, R.A. Porter and E.A. Oelke. (1995) Survival
of Zizania embryos in relation to moisture content, temperature
and maturity status. Seed Sci. Res. 5:31-40.
Walters-Vertucci, C., J. Crane and N.C. Vance. (1996) Physiological
aspects of Taxus brevifolia (Nutt.) seeds in relation to seed
storage characteristics. Physiol. Plant. 97:1-12.
Buitink, J., C. Walters, F.A. Hoekstra and J. Crane. (1998) Storage
behavior of Typha latifolia pollen at low water contents: Interpretation
on the basis of water activity and glass concepts. Physiol. Plant. 103:
145-153.
Walters, C., Pammenter, N.W., Berjak, P. and J. Crane. (2001) Desiccation
damage, accelerated aging and respiration in desiccation tolerant and
sensitive seeds. Seed Sci. Res. 11:135-148.
Crane, J., Miller, A.L., Van Roekel, J.W., and C. Walters. (2003) Triacylglycerols
determine the unusual storage physiology of Cuphea seed. Planta.
217:699-708.
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Patrick A. Reeves
(reevesp@lamar.colostate.edu)
Population genetics
and genomics
Education:
B.A. University of Colorado, Biology, 1992.
Research Interests:
Intraspecific phylogenetics
Molecular genetics of phenotypic evolution
Selected Publications:
Reeves, P.A., P.H. Friedman, and C.M. Richards. 2005. WolfPAC: building
a high-performance distributed computing network for phylogenetic analysis
using "obsolete" computational resources. Applied Bioinformatics
(in press).
Reeves, P. A., and R. G. Olmstead. 2003. Evolution of the TCP gene
family in Asteridae: cladistic and network approaches to understanding
regulatory gene family diversification and its impact on morphological
evolution. Molecular Biology and Evolution 20:1997-2009.
Olmstead, R. G., C. W. DePamphilis, A. D. Wolfe, N. D. Young, W. J.
Elisons, and P. A. Reeves. 2001. Disintegration of the Scrophulariaceae.
American Journal of Botany 88:348-361.
Reeves, P. A., and R. G. Olmstead. 1998. Evolution of novel morphological
and reproductive traits in a clade containing Antirrhinum majus (Scrophulariaceae).
American Journal of Botany 85:1047-1056.
Olmstead, R. G., P. A. Reeves, and A. C. Yen. 1998. Patterns of sequence
evolution and implications for parsimony analysis of chloroplast DNA.
In: Molecular Systematics of Plants II. D. E. Soltis, P. S. Soltis,
and J. J. Doyle, eds. pp.164-187.
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Ann A. Reilley
(reilley@lamar.colostate.edu)
Population genetics
and genomics
Education:
B.S. Texas A&M University, Plant and Soil Science, 1984
M.S. Texas A&M University, Plant Physiology, 1986
Research Interests:
Conservation of plant germplasm
Genetic diversity assessments using molecular markers
- Microsatellites (SSRs) for assessing conservation strategies for wildrice
- AFLPs and SSRs to determine effective population size and outcrossing
in collections of wild relatives of fruit crops (apple, pear, grape
and plum)
Plant tissue culture propagation & regeneration systems
Plant & bacterial transformation systems
Selected Publications:
Nissinen, R., F-M. Lai, M.J. Laine, P.J. Bauer, A.A. Reilley, X. Li,
S.H. De Boer, C.A. Ishimaru, and M.C. Metzler. 1997. Clavibacter
michiganenis subsp. sepedonicus elicits a hypersensitive response
in tobacco and secretes hypersensitive response-inducing protein(s).
Phytopathology 87:678-684.
Brown, S.E., A.A. Reilley, D.L.Knutdson, and C.A. Ishimaru. 2002. Genomic
fingerprinting of virulent and avirulent strains of Clavibacter
michiganensis subspecies sepedonicus. Current Microbiology 44:112-119.
Richards C.M., A.A. Reilley, D. Touchell, M.F. Antolin and C. Walters.
2004. A genetic analysis of cryogenic storage for ex situ conservation
of genetic diversity: A case study with Zizania texana. Conservation
Genetics 5:853-859.
Volk, G.M., C.M. Richards, A.A. Reilley, A.D. Henk, P.L. Forsline and
H.S. Aldwinckle. 2004. Ex situ conservation of vegetatively-propagated
species: Development of a seed base core collection for Malus
sieversii. Journal of the American Society for Horticultural
Science (in press, March issue).
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John W. Waddell
(jwaddell@lamar.colostate.edu)
Cryobiology of Vegetatively
Propagated Germplasm
Education:
B.A. University of Colorado, Boulder, Biology, 1972
M.F. Colorado State University, Forestry, 1981
Colorado State University, Horticulture (post-graduate studies)
Research Interests:
Cryopreservation of plant tissues
Plant cell biochemistry during acclimation and deacclimation (carbohydrates,
reactive oxygen species, antioxidant activity, lipid peroxidant)
Low temperature stress in plants
Differential Thermal Analyses, Thermo-Electric Module Emissions
Laboratory instrumentation and data acquisition
Other Activities:
Treasurer - Fort Collins Audubon Society
U.S. Naval Reserve, Retired
Selected Publications:
Weiser, R.L., S.J. Wallner, and J.W. Waddell. 1990. Cell wall and extension
mRNA changes during cold acclimation of pea seedlings. Plant Physiology.
93: 1021-1026.
Forsline, P.L., L.E. Towill, J.W. Waddell, C. Stushnoff, W.F. Lamboy,
and J.R McFerson. 1998. Recovery and longevity of cryopreserved dormant
apple buds. J. Amer. Soc. Hort. Sci. 123: 365-370.
Towill L.E., P.L. Forsline, C. Walters, J.W. Waddell and J. Laufmann.
2004. Cryopreservation of Malus germplasm using a winter vegetative
bud method: results from 1915 accessions. CryoLetters 25:323-334.
Back to top
Adam D. Henk (ahenk@lamar.colostate.edu)
Cellular
and molecular aspects of plant cryopreservation
Education:
BS Zoology, University of Georgia, 1996
Research Interests:
Assessments of genetic diversity of NPGS collections
Plant development and gene expression during cold acclimation
and cryopreservation protocols
Selected Publications:
Volk, G.M., C.M. Richards, A.A. Reilley, A.D. Henk, P.L. Forsline,
and H.S. Aldwinckle. 2005. Ex situ conservation of vegetatively-propagated
species: Development of a seed-base core collection for Malus sieversii.
Journal of the American Society for Horticultural Science (in press,
March issue).
Volk, G.M., A.D. Henk, and C.M. Richards. 2004. Genetic diversity among
US garlic clones as detected using AFLP methods. Journal of the American
Society for Horticultural Science 129:559-569.
Ashfield, T., A. Bocian, D. Held, et al. 2003. Genetic and physical
localization of the soybean Rpg1-b disease resistance gene reveals a
complex locus containing several tightly linked families of NBS-LRR
genes. Mol Plant Microbe In 16: 817-826.
Mizan, S., A. Henk, A. Stallings, et al. 2000. Cloning and characterization
of sialidases with 2-6 ' and 2-3 ' sialyl lactose specificity from Pasteurella
multocida J Bacteriol 182:6874-6883
Henk, A.D., R.F. Warren, and R.W. Innes. 1999. A new Ac-like transposon
of Arabidopsis is associated with a deletion of the RPS5 disease resistance
gene. Genetics 151: 1581-1589.
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Ann M.
Caspersen (ann.caspersen@ars.usda.gov)
Cellular and molecular
aspects of plant cryopreservation
Education:
B.S. Biology, 2000,University of Wyoming
M.S. Botany, 2004, University of Wyoming
Research Interests:
Cellular responses during cryoprotection
Cell ultrastructure
Protein function
Microscopy techniques
Selected Publications:
Caspersen, A.M., T.C. Vogelmann and A.W. Sylvester. 2001. Using cell
pattern mutants to explore the function of endoreduplication during
leaf development. Annual Maize Genetics Conference. 43:63.
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Dale R. Lockwood
(dale.lockwood@colostate.edu)
Population genetics
and genomics
Education:
B.S. New Mexico State University, Computer Science/Mathematics, 1987
M.S. University of Arizona, Applied Mathematics, 1992
M.S. University of California, Population Biology, 1999
Ph.D. University of California, Davis, Populatin Biology, 2002
Research Interests:
Gene flow, demographic dispersal and population dynamics
In situ and ex situ conservation strategies
Metapopulation ecology and genetics
Complexity in ecology
Selected Publications:
Wilen, J.E., M.D. Smith, D.R. Lockwood, and L.W. Botsford. 2002. Avoiding
surprises: Incorporating fishermen behaviour into management models.
Bulletin of Marine Science 70:553-575.
Lockwood, D.R., A. Hastings, and L.W. Botsford. 2002. The effects of
dispersal patterns on marine reserves: Does the tail wag the dog? Theoretical
Population Biology 61:297-309.
Lockwood, J.A., Narisu, S.P. Schell, and D.R. Lockwood. 2001. Canola
oil as a kairomonal attractant of rangeland grasshoppers (Orthoptera:
Acrididae): an economical liquid bait for insecticide formulation, Internat.
J. Pest Manage. 47:185-194.
Lockwood, D.R. and J.A. Lockwood. 1997. Evidence of self organized
criticality in insect populations. Complexity vol. 2, 4:49-58.
Lockwood, J.A. and D.R. Lockwood. 1993. A unified paradigm of rangeland
ecosystem dynamics through the application of catastrophe theory. J.
Range Manage. 46:282-288.
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Lana Wheeler (lanaw@lamar.colostate.edu)
Biophysical and
physiological aspects of seed development and quality
Education:
B.S. Horticulture, Colorado State University, 1997
Research Interests:
Cryopreservation of seeds
Longevity studies of various species
Historical studies regarding storage temperatures/conditions
Seed germination and vigor testing
Selected Publications:
Walters, C., L.J. Wheeler, and J. Grotenhuis. 2005. Longevity of seeds
stored in a genebank: species characteristics. Seed Science Research 15:1-20.
Walters, C., L.J. Wheeler, and P.C. Stanwood. 2004. Longevity of cryogenically-stored
seeds. Cryobiology 48:229-244.
Wiesner, L.E., J.E. Laufmann, P.C. Stanwood, and L.J. Wheeler. 1994.
The effect of liquid nitrogen on alfalfa seed viability, emergence, and
broken cotyledons. Journal of Seed Technology 18(1).
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Lisa M. Hill (lmhill@lamar.colostate.edu)
Biophysical and
physiological aspects of seed development and quality
Education:
B.S. Colorado State University, Microbiology, 1993
Research Interests:
Desiccation tolerance of dry biological systems
Plant embryo development and quality
Seed aging and volatile production
Conservation of genetic resources
Selected Publications:
Walters, C., L.M. Hill, and L.M. Wheeler. 2005. Dying while dry: kinetics
and mechanisms of deterioration in desiccated organisms. (in review)
Walters, C., and L.M. Hill. 1998. Water sorption of seeds from ultradry
experiments. Seed Science Research 8 (suppl):69-73.
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Remi M. Bonnart (remib@lamar.colostate.edu)
Cryobiology and
Microculture of Vegetatively Propagated Germplasm
Education:
B.S. Horticulture, Colorado State University, 2000
Research Interests:
Cryobiology of plant tissues
Cryopreservation of vegetatively propagated germplasm
Budding of dormant woody scions
In vitro propagation
Selected Publications:
Towill, L.E. and R.M. Bonnart. (in review). Cryopreservation of apple
using non-dessicated sections from winter-collected scions.
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