How tissue culture materials are placed into liquid nitrogen (LN2) long-term storage.

 
potato meristems growing in test tube tissue cultures
meristems in starpacks


Samples are received from field site sources.
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Information is given to the Information Systems Support group to assign identification numbers and print cards and labels.
Samples are tested for internal contamination.
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mint growing in tissue culture
Samples are grown out to get enough shoot tips to conduct LN2 exposure tests.
 
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mint plantlets in a petri dish

Testing is conducted to determine appropriate regimes, i.e. pretreatment procedures, vitrification, encapsulation, and/or dehydration, and retrieval methodology to assure 40%+ survival. (Tissue culture preservation in LN2)
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Success Failure
metal box used to store meristems in liquid nitrogen
Preserve 60+ viable shoot tips for long-term storage and 5-year monitor test. Retest, alter or develop new protocols.
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