ANNUAL  REPORT

Calendar Year 2002

 

 

 1.   NRSP-6: INTER-REGIONAL POTATO INTRODUCTION PROJECT

Introduction, Classification, Preservation, Evaluation and Distribution of tuber-bearing  Solanum Species.

 

2.   COOPERATIVE AGENCIES AND PRINCIPAL LEADERS

State Agricultural Experimental Stations                            

Representative

 

 

Southern Region                                             

J. C. Miller, Jr.

Western Region                                              Chairman (2003)

A. R. Mosley

North Central Region                                     Secretary (2003)

D. S. Douches  

Northeastern Region                       

 

 

United States Department of Agriculture

  

Agricultural Research Service

 

      Technical Representative                         Vice Chair (2003)

C. R. Brown

      National Program Staff

P. K. Bretting

      Area Director, Midwest Area

A. D. Hewings

Cooperative States Research Education

                   & Extension Service

A. M. Thro

Animal and Plant Health Inspection Service

L. E. Levy

Inter-Regional Potato Introduction Project    Project Leader

J. B. Bamberg

 

Agriculture Canada

T. R. Tarn  

 

Administrative Advisors

 

Southern Region

R. L. Westerman

Western Region

M. J. Burke

North Central Region                                      Lead

S. A. Slack

Northeastern Region

S. D. Reiling

                         


3.  PROGRESS AND PRINCIPAL ACCOMPLISHMENTS

 

 

A.  Introduction of New Stocks

 

Dr. Bamberg, accompanied by Charles Fernandez (US Potato Genebank); Dr. Chuck Brown (USDA, Prosser WA); and Dr. Joe Pavek (USDA, Retired), participated in a successful expedition to the southwest United States and obtained 19 new accessions for the collaborative intergenebank research project, and collected additional populations for screening of Columbia root-knot nematode.

 

A total of 73 seed accessions were assigned PI numbers in 2002: 16 from Russia, 27 from Poland, 11 from Bolivia, and 19 from the SW United States.  Six clonal accessions were assigned PI numbers in 2002: 4 from Mexico, 1 from Poland, and 1 from Bolivia. These accessions are now available from the NRSP-6 Solanum germplasm collection.

 

 

B.  Preservation and Increase of Stocks

 

In 2002, a total of 91 accessions were increased as botanical seed populations.

 

A total of 600 potato spindle tuber viroid (PSTV) tests were performed on seed increase parents, seed lots and research materials. Germination tests were performed on 1,106 accessions; ploidy determinations were made on 30 accessions.

 

Research continues on the use of 2,4-D on pollinated flowers to help with fruit retention and seed production.  Growth retardants are being assessed for use as tuber and flower promoters in the wild species and as a method to control plant height in greenhouse and screenhouse-grown plants for crossing.

 

The Association of Potato Intergenebank Collaborators (APIC) constructed a database of all wild potato holdings of the potato genebanks in Argentina, Europe, Peru, and the United States.  The Intergenebank Potato Database (IPD) (www.potgenebank.org/ipd/) continues to be a very useful resource for clientele around the world.  Dr. Alfonso del Rio represented NRSP-6 at the APIC meeting in Hamburg, Germany.

 

A study was completed comparing germplasm from the Russian (VIR) and United States (NRSP-6) “duplicate” accessions.  Most are significantly different.  Work continues on comparisons between US and CIP collections.  The next step in the study will be to look at DNA comparisons to variation in trait expression or frequency between intergenebank seedlots.

  

Work continued with Dr. C. Miller’s lab screening for antioxidants in uncolored wild potato species’ tubers.  Previous screening among species identified ones with promising levels of tuber antioxidant capacity.  Some diploid “Mexican” species seem to have high antioxidant in common:  S. cardiophyllum, S. jamesii, and S. pinnatisectum.  These and other species were retested with multiple PIs.  Variation within promising PIs was also studied.  Only S. pinnatisectum demonstrated particularly consistent high levels within populations.

 

Cold resistant breeding work continues with recurrent selection of S. tuberosum, S. acaule and S. commersonii hybrids.  Materials are now maturing earlier with the introgression of very early varieties and which survive in vitro leaf freezing to –5oC.  Selections were planted at Copic Bay, Oregon area in the summer of 2002.  The best selections will be submitted to UW Madison colleagues for more focused lab assessments.

 

Late Blight screening at a field site very close to Lake Michigan (cool temperatures and high humidity) was completed for a second year.  In 2001, two highly segregating PIs of S. microdontum 473170 and S. okadae 458367 and their F2 hybrids were tested.  There was a 70% correlation with previous results on the same clones at Cornell by Bill Fry, so this local test will be useful to verify resistance of newly imported germplasm.  In 2002, Polish, Russian, Mexican and CIP introductions were tested.

 

Geographical parameters and proximity to related species predict genetic variation in the inbred potato species Solanum verrucosum.  Such links had not been previously detected in S. fendleri, S. jamesii or S. sucrense.  Resolution and fine-tuning to locations may be enhanced by inbreeding and uniformity.  The highest correlations to genetic rarity (a population’s average genetic difference from all others) were latitude, longitude and proximity to the nearest S. hjertingii population.

 

 

C.  Classification

 

Dr. Spooner continues to resolve problems in taxonomic classification that impede both efficient documentation and use of the germplasm. At present Dr. Spooner, in collaboration with Dr. R van den Berg, Dr. R. Hijmans, and Dr. A. Rodriguez, is working on a book of Wild Potato Species of North and Central America, which will be a valuable tool for germplasm users worldwide.

 

 

D.  Distribution

 

NRSP-6 distributed 3,447 units of seed, 643 tuber families, 919 in vitro stocks and 215 herbarium samples to clientele in 20 states of the United States and 14 other countries.  Internally, NRSP-6 used 6,371 units of seed for chromosome counts, germination tests, identification and taxonomic check plantings, in vitro maintenance, seed increases, PSTV tests, and miscellaneous plantings.  The volume and types of stocks sent to various consignee categories are summarized in the table below.

 

  

VOLUME AND TYPES OF STOCKS DISTRIBUTED

 

 

                                 Units1

 

Category

     S

  TF

 TS

IVS

HERB

TOTAL

    PIs

Domestic

  3,165

  613

 186

583

  215

     4,762

  4,074

Foreign

     282

    30

 293

336

      0

        941

     704

NRSP-62

  6,367

      0

     1

    3

      0

     6,371

  2,384

Total

  9,814

  643

 480

922

  215

   12,074

  7,162

1 Types of stocks sent/(number of seeds, tubers or plantlets per standard shipping unit):    S= True Seeds/(50),

  TF= Tuber Families/(10),  TS=Tuber Stocks/(3), IVS=In Vitro Stocks/(1), Herbarium/(1).

2 Includes chromosome counts, germination tests, ID and Taxonomic check plantings, in vitro maintenance, seed

  increases, PSTV tests, miscellaneous plantings, and NSSL seed backup.

 

 

 

G.  Visitors From Other Countries

 

     Ms N. Gomez                 Panama

     Dr. P. Anderson              Peru

     Dr. J. Prohens                 Spain 

     Dr. Y. Hosaka                Japan

 

 

 

4.  USEFULNESS OF FINDINGS

 

NRSP-6's purpose is to provide a ready source of raw materials, technology and information to support potato enhancement, breeding and research in the US and around the world.  Thus, one way the success of NRSP-6 can be measured is by the use of NRSP-6 germplasm in the pedigrees of new, improved potato cultivars.  Another is in the use of NRSP-6 stocks in more basic research programs, which also ultimately contribute to human utilization of the potato crop, these being reflected in publications.

 

Five cultivar releases were published in the American Journal of Potato Research in 2002: ‘AC Sunbury’, ‘IdaRose’, ‘Red Companion’, ‘Gem Russet’, and ‘Bannock Russet’.  All are known to have wild species in their pedigrees.

 

Section 6 lists 124 papers, 32 abstracts, 1 poster, and 3 theses which report the use of NRSP-6 Solanum introductions this year.

 

 

 5.  WORK PLANNED FOR 2003

 

Evaluation experiments will be continued on Solanum species for the following traits: antioxidants, frost hardiness, rooting vigor, tuber calcium, late blight resistance, hormone mutants, and glycoalkaloids.

 

The general objective of NRSP-6, to promote and facilitate potato research and breeding, will be pursued by continuing high quality service with respect to introduction, preservation, classification, evaluation, and distribution of potato germplasm to clients in the U.S. and around the world.

 

APIC intergenebank projects researching the status and dynamics of genetic diversity in the collection using RAPDs and strengthening ties with sister genebanks around the world will be continued.

  

 

 6. PUBLICATIONS ISSUED DURING THE YEAR

 

 

A.     Publications issued by NRSP-6 Personnel

 

del Rio, A.H. and J.B. Bamberg.  2002.  Lack of association between genetic and geographic origin characteristics for the wild potato Solanum sucrense Hawkes.  Am. J. Potato Res. 79:335-338.

 

Hijmans, R.J., D.M. Spooner, A.R. Salas, A. Guarino, and J. de la Cruz.  2002.  Atlas of wild potatoes.  Systematic and Ecogeographic Studies on Crop Genepools, International Plant Genetic Resources Institute, Rome 10:i-ix, 1-129. 

 

Huaman, Z. and D.M. Spooner.  2002.  Reclassification of landrace populations of cultivated potatoes (Solanum sect. Petota).  Am. J. Bot. 89:947-965.

 

Raker, C. and D.M. Spooner.  2002.  The Chilean tetraploid cultivated potato, Solanum tuberosum, is distinct from the Andean populations; microsatellite data.  Crop Sci. 42:1451-1458.

 

Rodriguez, A. and D.M. Spooner.  2002.  Subspecies boundaries of the wild potatoes Solanum bulbocastanum and S. cardiophyllum based on morphological and nuclear RFLP data.  Acta Mexicana 61:9-25.

 

Spooner, D.M.  2002.  Strengths and weaknesses of molecular markers for studies of diversity in genebank holdings.  In: Proceedings of the International Workshop on: Aplicaciones de los marcadores moleculares en la conservacion y manejo de la agrobiodiversidad, International Potato Center, CIP, Lima, Peru.

 

Spooner, D.M., R.G. van den Berg, G. Bryan, and A. del Rio.  2002.  Reduction of species in the wild potato Solanum section Petota series Longipedicellata: AFLP, RAPD and chloroplast SSR data.  XXVIth International Horticultural Congress, Toronto, ON, Canada, August 11-17, 2002, pp. 114-115.  (Abstract)

 

van den Berg, R.G., G. Bryan, A. del Rio, and D.M. Spooner.  2002.  Reduction of species in the wild potato Solanum section Petota series Longipedicellata: AFLP, RAPD and chloroplast SSR data.  Theor. Appl. Genet. 105:1109-1114.

 

B.    Journal Articles and Abstracts Reporting Research with NRSP-6 Stocks

 

Adams, J.S.  2002.  Banking on Genes: Preserving the traits of today for the potato of tomorrow.  Potato Grower (November): 26-28.

 

Ahloowalia, B.S.  2000.  Global conference on potato, New Delhi, India, 6-11 December 1999.  AgBiotechNet 2(ABN049):1-4.

 

Ahn YulKyun, Kim HeiYoung, Choi HakSun, Kim KiTaek, and Park HyoGuen.  2001.  Production of interspecific somatic hybrids between the cultivated potato (Solanum tuberosum) and the wild species (S. brevidens).  J. Korean Soc. Hort. Sci. 42(4):420-424.

 

Ahn YulKyun, Sin JiYoung, Lee DongWoo, Om YoungHyun, and Park HyoGuen.  2001.  Characteristics of interspecific somatic hybrids between Solanum brevidens and S. tuberosum (‘Superior’, ‘Dejima’, and dihaploid of ‘Superior’).  J. Korean Soc. Hort. Sci. 42(4):425-429.

 

Ahn YulKyun, Kang JuChang, Kim HeiYoung, Lee SeungDon, and Park HyoGuen.  2001.  Resistance to blackleg and tuber soft rot in interspecific somatic hybrids between S. brevidens and  S. tuberosum (‘Superior’, ‘Dejima’, and dihaploid of ‘Superior’).  J. Korean Soc. Hort. Sci. 42(4):430-434.

 

Ahn YulKyun, Bang JaeWook, Choi GookSeoun, and Park HyoGuen.  2001.  Introgression of resistance to PLRV and PVY from Solanum brevidens into S. tuberosum (‘Superior’, ‘Dejima’, and dihaploid of ‘Superior’) by interspecific somatic hybrids.  J. Korean Soc. Hort. Sci. 42(6):707-711.

 

Ahn Yul-Kyun, Bo-Sun Kim, Jae-Wook Bang, and Hyo-Guen Park.  2002.  Cytogenetic analysis of somatic hybrids between Solanum brevidens and S. tuberosum (‘Superior’, ‘Dejima’, and dihaploid of ‘Superior’) by GISH.  XXVIth International Horticultural Congress, Toronto, ON, Canada, August 11-17, 2002, p. 110.  (Abstract)

 

Anjum, M.A.  2001.  Cytology of potato callus cells in relation to their frost hardiness.  Biologia Plantarum 44(3):325-331.

 

Ashouri, A., D. Michaud, and C. Cloutier.  2001.  Recombinant and classically selected factors of potato plant resistance to the Colorado potato beetle, Leptinotarsa decemlineata, variously affect the potato aphid parasitoid Aphidius nigripes.  Biocontrol 46(4):401-418.

 

Audy, Patrice, Richard Tarn, and Agnes Murphy.  2002.  Identification of a differential gene expression pattern related to resistance to potato late blight.  XXVIth International Horticultural Congress, Toronto, ON, Canada, August 11-17, 2002, pp. 110-111.  (Abstract)

 

Barone, A., J. Li, A. Sebastiano, T. Cardi, and L. Frusciante.  2002.  Evidence for tetrasomic inheritance in a tetraploid Solanum commersonii (+) S. tuberosum somatic hybrid through the use of molecular markers.  Theor. Appl. Genet. 104(4):539-546.

 

Ballvora, A., M.R. Ercolano, J. Weiss, K. Meksem, C.A. Bormann, P. Orberhagemann, F. Salamini, and C. Gebhardt.  2002.  The R1 gene for potato resistance to late blight (Phytophthora infestans) belongs to the leucine zipper/NBS/LRR class of plant resistance genes.  Plant J. 30(3):361-371.

 

Bisognin, D.A., D.S. Douches, K. Jastrzebski, and W.W. Kirk.  2002.  Half-sib progeny evaluation and selection of potatoes resistant to the US8 genotype of Phytophthora infestans from crosses between resistant and susceptible parents.  Euphytica 125(1):129-138.

 

Boluarte-Medina, T. and R.E. Veilleux.  2002.  Phenotypic characterization and bulk segregant analysis of anther culture response in two backcross families of diploid potato.  RAPD markers for androgenesis in potato.  Plant Cell, Tissue and Organ Culture 68(3):277-286.

 

Boluarte-Medina, T., E. Fogelman, E. Chani, A.R. Miller, I. Levin, D. Levy, and R.E. Veilleux.  2002.  Identification of molecular markers associated with leptine in reciprocal backcross families of diploid potato.  Theor. Appl. Genet. 105:1010-1018.

 

Bonierbale, M., J. Landeo, and M. Ghislain.  2002.  Targeted breeding for sustainable production in a global potato improvement.  XXVIth International Horticultural Congress, Toronto, ON, Canada, August 11-17, 2002, p. 110.  (Abstract)

 

Bradeen, J.M., S.K. Naess, J. Song, S.M. Wielgus, and G.T. Haberlach.  2002.  Analysis of 300kb of genomic sequence associated with late blight resistance of potato.  XXVIth International Horticultural Congress, Toronto, ON, Canada, August 11-17, 2002, p. 117.  (Abstract)

 

Bradshaw, J.E., A.K. Lees, and H.E. Stewart.  2000.  How to breed potatoes for resistance to fungal and bacterial diseases.  Pl. Breed. Seed Sci. 44(2):3-20.

 

Brown, C.R., H. Mojtahedi, and G.S. Santo.  2002.  Characteristics of resistance to Columbia root-knot nematode introgressed from several Mexican and North American wild potato species.  XXVIth International Horticultural Congress, Toronto, ON, Canada, August 11-17, 2002, p. 116.  (Abstract)

 

Bryan, G.J., K. McLean, J.E. Bradshaw, W.S. de Jong, M. Phillips, L. Castelli, and R. Waugh.  2002.  Mapping QTLs for resistance to the cyst nematode Globodera pallida derived from the wild potato species Solanum vernei.  Theor. Appl. Genet. 105(1):68-77.

 

Bu QingYun, Lu WenHe, Chen YiLi, Tian XingYa, Wang FengYi, and Qin Xin.  2002.  Evaluation of some newly developed diploid hybrids and their breeding value in 4x-2x crosses.  J. NE Ag. Univ. (English Edition) 9(1):35-41.

 

Capo, A., M. Cammareri, F. Della Rocca, A. Errico, A. Zoina, and C. Conicella.  2002.  Evaluation for chipping and tuber soft rot (Erwinia carotovora) resistance in potato clones from unilateral sexual polyploidization (2x x 4x).  Am. J. Potato Res. 79:139-145.

 

Cardi, T., M. Mazzei, and L. Frusciante.  2000.  Multivariate analysis of variation in the field of tetraploid and (hypo)-hexaploid Solanum commersonii (+) S. tuberosum somatic hybrids.  Italian J. Agronomy 4(2):67-74.

 

Cardi, T., M. Mazzei, and L. Frusciante.  2002.  Field variation in a tetraploid progeny derived by selfing a Solanum commersonii (+) S. tuberosum somatic hybrid: a multivariate analysis.  Euphytica 124(1):111-119.

 

Carputo, D., A. Barone, S. Carbone, and L. Frusciante.  2001.  Innovative breeding techniques for early potato production.  Sementi Elette 47(4):23-27.

 

Carputo, D., L. Frusciante, L. Monti, M. Parisi, and A. Barone.  2002.  Tuber quality and soft rot resistance of hybrids between Solanum tuberosum and the incongruent wild relative S. commersonii.  Am. J. Potato Res. 79:345-352.

 

Chani, E., V. Ashkenazi, J. Hillel, and R.E. Veilleux.  2002.  Microsatellite marker analysis of an anther-derived potato family: skewed segregation and gene-centromere mapping.  Genome 45:236-242.

 

Christ, B.J., K.G. Haynes, and B.T. Vinyard.  2002.  Inheritance of early blight resistance from open-pollinated 4x-2x potato hybrids.  Am. J. Potato Res. 79:403-410.

 

Coffin, R.H., J. Coffin, R. Singh, X. Nie, and R.Y. Yada.  2002.  Potato breeding at the cross roads – options to consider.  XXVIth International Horticultural Congress, Toronto, ON, Canada, August 11-17, 2002, pp. 115-116.  (Abstract)

 

Collins, W.W.  2000.  The Global Initiative on Late Blight – alliance for the future. In: Potato, global research & development.  Proc. Global Conf. On Potato, New Delhi, India, 6-11 December 1999, Vol 1.  [ S.M.P. Khurana, G.S. Shekhawat, B.P. Singh, and S.K. Pandey, (Eds.)].  Shimla, India; Indian Potato Assoc., p. 513-524.

 

Collins, W.W.  2001.  Conservation and utilization of biodiversity in the Andean ecoregion.  In Crop Science: Progress and prospects.  Third International Crop Science Congress, Hamburg, Germany, August 17-22, 2000.  CABI Publishing: 231-242.

 

Cook, R. and G.R. Noel.  2002.  Cyst nematodes: Globodera and Heterodera species.  In: Plant resistance to parasitic nematodes [J.L. Starr, R. Cook, and J. Bridge, Eds.].  CABI Publishing 71-105.

 

Coombs, J.J., D.S. Douches, Li Wenbin, E.J. Grafius, and W.L. Pett.  2002.  Combining engineered (Bt-cry3A) and natural resistance mechanisms in potato for control of Colorado potato beetle.  J. Am. Soc. Hort. Sci. 127(1):62-68.

 

Costanzo, S., B.J. Christ, and K.G. Haynes.  2002.  Construction of a molecular linkage map of diploid potato hybrid (Solanum phureja x S. stenotomum) for identification of QTLs involved in late blight resistance.  Presented at 86th Annual PAA Meeting, XXVIth International Horticultural Congress, Toronto, ON, Canada, August 11-17, 2002.  (Abstract)

 

Culley, D.E., B.B. Dean, and C.R. Brown.  2002.  Introgression of the low browning trait from the wild Mexican species Solanum hjertingii into cultivated potato (S. tuberosum L.).  Euphytica 125(3):293-303.

 

Darsow, U.  2000.  Sources of and breeding for relative late blight resistance of potato.  In: Potato, global research & development.  Proc. Global Conf. On Potato, New Delhi, India, 6-11 December 1999, Vol 1.  [ S.M.P. Khurana, G.S. Shekhawat, B.P. Singh, and S.K. Pandey, (Eds.)].  Shimla, India; Indian Potato Assoc., p. 561-570.

 

Davies, C.S., M.J. Ottman, and S.J. Peloquin.  2002.  Can germplasm resources be used to increase the ascorbic acid content of stored potatoes?  Am. J. Potato Res. 79:295-299.

 

DeJong, W., N. Eannetta, D. DeJong, M. Bodis, and Chun Suk Jung.  2002.  Candidate gene analysis of loci controlling natural variation for potato skin color.  XXVIth International Horticultural Congress, Toronto, ON, Canada, August 11-17, 2002, p. 111.  (Abstract)

 

Dinu, I.I. and C.A. Thill.  2002.  Cytological analysis of a S. pinnatisectum – S. tuberosum bridging genotype developed by EBN manipulation and sexual crosses. Presented at 86th Annual PAA Meeting, XXVIth International Horticultural Congress, Toronto, ON, Canada, August 11-17, 2002.  Am. J. Potato Res.: In Press.  (Abstract)

 

Douches, D., K. Felcher, J. Coombs, and W. Kirk.  2002.  Field evaluation of foliar resistance to Phytophthora infestans in potato.  XXVIth International Horticultural Congress, Toronto, ON, Canada, August 11-17, 2002, p. 117.  (Abstract)

 

Evtushenko, D.P., A.M. Shakhovskii, and V.A. Sidorov.  2001.  Production of potato cybrids without using genetic selectable markers of the parental material: Solanum tuberosum L. plants (cv. Svitanok Kievskii) with S. pinnatisectum Dun. plastids.  Tsitologiya I Genetika 35(6):3-9.

 

Fisher, D.G., K.L. Deahl, and M.V. Rainforth.  2002.  Horizontal resistance in Solanum tuberosum to Colorado potato beetle (Leptinotarsa decemlineata Say).  Am. J. Potato Res. 79:281-293.

 

Fock, I., C. Collonnier, J. Luisetti, A. Purwito, V. Souvannavong, F. Vedel, A. Servaes, A. Ambroise, H. Kodja, G. Ducreux, and D. Sihachakr.  2001.  Use of Solanum stenotomum for introduction of resistance to bacterial wilt in somatic hybrids of potato.  Pl. Phys. Biochem. 39(10):899-908.

 

Fu Hongyong, Du Ji, Song Junqi, Jiang Jiming, and W.D. Park.  2001.  Potato and tomato Forever Young genes contain class-I patatin promoter-like sequences.  Botanical Bulletin of Academia Sinica 42(4):231-241.

 

Garry, G., G. Forbes, A Salas, W. Perez, M. Santa Cruz, H.M. Pinedo, E. Gonzales, M. Rivera, and R.J. Nelson.  2001.  Characterization of Phytophthora infestans colonizing different Solanaceous species in Peru, with implications on the control of potato late blight.  International Potato Center, Lima, Peru, Scientist and farmer: partners in research for the 21st Century, Program Report 1999-2000, 39-47.

 

Gauer, P.C., J. Gopal, S.K. Pandey, and M.S. Rana.  2002.  EX/A680-16 (reg. No. INGR 01011) potato.  Indian J. Genet. Pl. Breed. 62(1):94.

 

Ghislain, M., F. Rodriguez, F. Villamon, J. Nunez, R. Waugh, and M. Bonierbale.  2001.  Establishment of microsatellite assays for potato genetic identification.  International Potato Center, Lima, Peru, Scientist and farmer: partners in research for the 21st Century, Program Report 1999-2000, 167-174.

 

Golmirzaie, A. and R. Ortiz.  2002.  Inbreeding and true seed in tetrasomic potato.  III.  Early selection for seedling vigor in open-pollinated populations.  Theor. Appl. Genet. 104(1):157-160.

 

Gopal, J.  2000.  Genetic analysis for family selection in potato breeding programs.  In: Potato, global research & development.  Proc. Global Conf. On Potato, New Delhi, India, 6-11 December 1999, Vol. 1.  [ S.M.P. Khurana, G.S. Shekhawat, B.P. Singh, and S.K. Pandey, (Eds.)].  Shimla, India; Indian Potato Assoc., p. 138-142.

Gopal, J.  2001.  Genetic analysis of within family variation in true seed crop of potato (Solanum tuberosum L.).  Indian J. Genet. Pl. Breed.  61(2):147-150.

 

Groza, H.I., B.D. Bowen, D. Kichefski, S.J. Peloquin, and J. Jiang.  2002.  Red Companion: A new red potato variety for fresh market.  Am. J. Potato Res. 79:133-137.

 

Grunwald, N.J., W.G. Flier, A.K. Sturbaum, E. Garay-Serrano, T.B.M. van den Bosch, C.D. Smart, J.M. Matuszak, H. Sozoya-Saldana, L.J. Turkensteen, and W.E. Fry.  2001.  Population structure of Phytophthora infestans in the Toluca Valley region of  Central Mexico.  Phytopathology 91(9):882-890.

 

Grunwald, N.J., M.A. Cadena Hinojosa, O. Rubio Covarrubias, A. Rivera Pena, J.S. Niederhauser, and W.E. Fry.  2002.  Potato cultivars from the Mexican National Program: sources and durability of resistance against late blight.  Phytopathology 92(7):688-693. 

 

Hayes, R.J. and C.A. Thill.  2002.  Co-current introgression of economically important traits in a potato-breeding program.  Am. J. Potato Res. 79:173-181.

 

Hayes, R.J. and C.A. Thill.  2002.  Introgression of cold (4 C) chipping from 2x (2 Endosperm Balance Number) potato species into 4x (4EBN) cultivated potato using sexual polyploidization.  Am. J. Potato Res. 79:421-431.

 

Hayes, R.J. and C.A. Thill.  2002.  Selection for cold chipping genotypes from three early generations in a potato breeding program.  Euphytica 128:353-362.

 

Hayes, R.J. and C.A. Thill.  2002.  Selection for potato genotypes from diverse progenies that combine 4 C chipping with acceptable yields, specific gravity and tuber appearance.  Crop Sci. 42:1343-1349.

 

Hayes, R.J. and C.A. Thill.  2002.  Early generation selection to rapidly develop cold chipping germplasm.  Presented at 86th Annual PAA Meeting, XXVIth International Horticultural Congress, Toronto, ON, Canada, August 11-17, 2002, p. 121. (Abstract)

 

Haynes, K.G., B.J. Christ, P.D. Weingartner, D.S. Douches, C.A. Thill, G. Secor, W.E. Fry, and D.H. Lambert.  2002.  Foliar resistance to late blight in potato clones evaluated in national trials in 1997.  Am. J. Potato Res. 79:451-457.

 

Helgeson, J.P. and G.T. Haberlach.  2002.  Useful lessons learned from somatic hybrids.  XXVIth International Horticultural Congress, Toronto, ON, Canada, August 11-17, 2002, p. 122.  (Abstract)

 

Horvath, J. and G. Kazinczi.  2000.  Potato virus research in Hungary: a short history with international aspects.  In: Potato, global research & development.  Proc. Global Conf. On Potato, New Delhi, India, 6-11 December 1999, Vol 1.  [ S.M.P. Khurana, G.S. Shekhawat, B.P. Singh, and S.K. Pandey, (Eds.)].  Shimla, India; Indian Potato Assoc., p. 304-321.

 

Horvath, J., A. Takacs, and G. Kazinczi.  2001.  Solanum stoloniferum and S. tarnii as resistance sources of the NTN strain of potato Y potyvirus (PVYNTN).  In: Proc. 5th Slovenian Conf. Plant Protection, Catez ob Savi, Slovenia, 6-8 March 2001.   p. 227-231.

 

Hosaka, Kazuyoshi.  2002.  Distribution of the 241 bp deletion of chloroplast DNA in wild potato species.  Am. J. Potato Res. 79-119-123.

 

Jansky, S.H. and D.I. Rouse.  2002.  Variability for disease resistance in populations of potato haploids.  XXVIth International Horticultural Congress, Toronto, ON, Canada, August 11-17, 2002, p. 118.  (Abstract)

 

Kaushik, S.K., P.H. Singh, B.P. Singh, J. Gopal, and R.K. Birhman.  2000.  Late blight resistance in Solanum tuberosum germplasm.  In: Potato, global research & development.  Proc. Global Conf. On Potato, New Delhi, India, 6-11 December 1999, Vol 1.  [ S.M.P. Khurana, G.S. Shekhawat, B.P. Singh, and S.K. Pandey, (Eds.)].  Shimla, India; Indian Potato Assoc., p. 603-606.

 

Kimura, T. and K. Hosaka.  2002.  Genetic mapping of a dwarfing gene found in Solanum phureja clone 1.22.  Am. J. Potato Res. 79:201-204.

 

Kiru, S.D.  2000.  Sources of resistance to late blight among Andigena accessions.  In: Potato, global research & development.  Proc. Global Conf. On Potato, New Delhi, India, 6-11 December 1999, Vol. 1.  [ S.M.P. Khurana, G.S. Shekhawat, B.P. Singh, and S.K. Pandey, (Eds.)].  Shimla, India; Indian Potato Assoc., p. 578-582.

 

Kuhl, J.C., M.J. Havey, and R.E. Hanneman, Jr.  2002.  A genetic study of unilateral incompatibility between diploid (1EBN) Mexican species Solanum pinnatisectum and S. cardiophyllum subsp. cardiophyllum.  Sexual Plant Reproduction 14(6):305-313.

 

Landeo, J.A., M. Gastelo, G. Beltran, and L. Diaz.  2001.  Quantifying genetic variance for horizontal resistance to late blight in potato breeding population B3C1.  International Potato Center, Lima, Peru, Scientist and farmer: partners in research for the 21st Century, Program Report 1999-2000, 63-68.

 

Laurila, J., I. Laakso, J. Larkka, T. Gavrilenko, V.M. Rokka, and E. Pehu.  2001.  The proportions of glycoalkaloid aglycones are dependent on the genome constitutions of interspecific hybrids between two Solanum species (S. brevidens and S. tuberosum).  Plant Science 161(4):677-683.

 

Laurila, J., J. Larkka, A. Tauriainen, V.M. Rokka,. E. Pehu, I. Laakso, and T. Gavrilenko.  2001.  Variation in proportions of glycoalkaloid aglycones in somatic hybrids between two potato species (Solanum tuberosum and S. brevidens) possessing different genome constitutions.  In: Proc. 4th Internatl. Symposium on In Vitro Culture and Hort. Breeding, Tampere, Finland, 2-7 July 2000.  [S. Sorvari, S. Karhu, E. Kanervo, and S. Pihakashi, (Eds.)].  Acta Hort. 560:173-176.

 

Levy, D.  2000.  Achievements in potato breeding in diverse climates.  In: Potato, global research & development.  Proc. Global Conf. On Potato, New Delhi, India, 6-11 December 1999, Vol 1.  [ S.M.P. Khurana, G.S. Shekhawat, B.P. Singh, and S.K. Pandey, (Eds.)].  Shimla, India; Indian Potato Assoc., p. 122-128.

 

Liedberg, J. and J. Lorenzen.  2002.  Resistance to PLRV and PVY in progeny of S. tuberosum fusions.  XXVIth International Horticultural Congress, Toronto, ON, Canada, August 11-17, 2002, p. 118.  (Abstract)

 

Lightbourn, G. and R.E. Veilleux.  2002.  Retrotransposon based markers to characterize somatic hybrids and assess variation induced by protoplast fusion of monoploid potato.  XXVIth International Horticultural Congress, Toronto, ON, Canada, August 11-17, 2002, p. 112.  (Abstract)

 

Lim, Hak-Tae, Kuihua Li, and Yilan Fang.  2002.  Development of potato varieties as healthy foods with high biological function.  XXVIth International Horticultural Congress, Toronto, ON, Canada, August 11-17, 2002, pp. 133-134.  (Abstract)

 

Love, S.L., R. Novy, D.L. Corsini, J.J. Pavek, A.R. Mosley, R.E. Thornton, S.R. James, and D.C. Hane.  2002.  Gem Russet: A long russet potato variety with excellent fresh market and french fry processing quality.  Am. J. Potato Res. 79:25-31.

 

Love, S.L., D.L. Corsini, R. Novy, J.J. Pavek, A.R. Mosley, R.E. Thornton, S.R. James, D.C. Hane, and K.A. Rykbost.  2002.  IdaRose: A potato variety with bright red skin, excellent culinary quality, and long tuber dormancy.  Am. J. Potato Res. 79:79-84.

 

Love, S.L., T. Salaiz, B. Shafii, W.J. Price, A.R. Mosley, and R.E. Thornton.  2002.  Ascorbic acid content and stability in North American potato germplasm.  XXVIth International Horticultural Congress, Toronto, ON, Canada, August 11-17, 2002, p. 115.  (Abstract)

 

Lozoya-Saldana, H., A. Hernandez-Vilchis, E. Garay-Serrano, C.R. Brown, N. Grunwald, and J.P. Helgeson.  2001.  Genetic studies and breeding for stable late blight resistance of potato in the United State and Mexico.  Revista Mexicana de Fitopatologia 19(2):253-259.

 

Lu WenHe, K. Haynes, and E. Wiley.  2001.  Carotenoid content and color in diploid  potatoes.  J. Am. Soc. Hort. Sci. 126(6):722-726.

 

Lu, W., K. Haynes, E. Wiley, and B. Clevidence.  2002.  Carotenoid contents in leaves and tubers of yellow-fleshed diploid potatoes.  XXVIth International Horticultural Congress, Toronto, ON, Canada, August 11-17, 2002, p. 112.  (Abstract)

 

Luu DoanTrung, Qin Xike, G. Laublin, Yang Qing, D. Morse, and M. Cappadocia.  2001.  Rejection of S-heteroallelic pollen by a dual-specific S-RNase in Solanum chacoense predicts a multimeric SI pollen component.  Genetics 159(1):329-335.

 

Maine, M.J. de, A.K. Lees, D.D. Muir, J.E. Bradshaw, and G.R. Mackay.  2000.  Long-day-adapted Phureja as a resource for potato breeding and genetic research.   In: Potato, global research & development.  Proc. Global Conf. On Potato, New Delhi, India, 6-11 December 1999, Vol 1.  [ S.M.P. Khurana, G.S. Shekhawat, B.P. Singh, and S.K. Pandey, (Eds.)].  Shimla, India; Indian Potato Assoc., p. 134-137.

 

Manosalva, P., S. Torres, F. Trognitz, R. Gysin, D. NinoLiu, R. Simon, M. Herrera, W. Perez, J. Landeo, B. Trognitz, M. Ghislain, and R. Nelson.  2001.  Plant defense genes associated with quantitative resistance to potato leaf blight.  International Potato Center, Lima, Peru, Scientist and farmer: partners in research for the 21st Century, Program Report 1999-2000, 27-37.

 

Markarov, A.M.  2002.  Causes of flowering of long-day potato species under short-day and cold-night conditions.  Russian J. Plant Phys. 49(4):465-469.

 

Martinez-Villegas, E., J. Kohashi-Shibata, E. Garcia-Moya, and J.A.S. Escalante-Estrada.  2000.  Growth and yield of wild potato (Solanum cardiophyllum Lindl.) in relation to thermoperiod and photoperiod.  Ag. Tec. Mexico 26(2):183-190.

 

McCue, K.F., D.L. Corsini, L.V.T. Shepherd, C.P. Moehs, P. Joyce, H. Davies, and W.R. Belknap.  2002.  Reduction of total steroidal glycoalkaloids in potato tubers using antisense constructs of a gene encoding a solanidine glucosyl transferase.  XXVIth International Horticultural Congress, Toronto, ON, Canada, August 11-17, 2002, p. 115.  (Abstract)

 

McGrath, J.M., C.E. Williams, G.T. Haberlach, S.M. Wielgus, T.F. Uchytil, and J.P. Helgeson.  2002.  Introgression and stabilization of Erwinia  soft rot resistance into potato after somatic hybridization of Solanum tuberosum and S. brevidens.  Am. J. Potato Res. 79:19-24.

 

McGregor, C.E., R. van Treuren, R. Hoekstra, and T.J.L. van Hintum.  2002.  Analysis of the wild potato germplasm of the series Acaulia with AFLPs: implications for ex situ conservation.  Theor. Appl. Genet. 104(1):146-156.

 

Mihovilovich, E., L. Alarcon, A.L. Perez, and M. Bonierbale.  2001.  Discovery and evaluation of a valuable new source of resistance to PLRV: Solanum tuberosum subsp. andigena.  International Potato Center, Lima, Peru, Scientist and farmer: partners in research for the 21st Century, Program Report 1999-2000, 95-103.