ANNUAL  REPORT

Calendar Year 2001

1.   NRSP-6: INTER-REGIONAL POTATO INTRODUCTION PROJECT    

Introduction, Classification, Preservation, Evaluation and Distribution of tuber-bearing  Solanum Species.

 2.   COOPERATIVE AGENCIES AND PRINCIPAL LEADERS

United States Department of Agriculture 

Administrative Advisors

3.  PROGRESS AND PRINCIPAL ACCOMPLISHMENTS

A.  Introduction of New Stocks

Dr. Bamberg, accompanied by Charles Fernandez (US Potato Genebank), participated in a successful expedition to the southwest United States and obtained ten new accessions for the collaborative intergenebank research project.

A total of 34 seed accessions were assigned PI numbers in 2001: 13 from Russia, 10 from Peru, 1 from Mexico, and 10 from the SW United States.  51 clonal accessions were assigned PI numbers in 2001, 3 from Mexico, 18 from Peru, 9 from Poland, and 20 from Sweden. These accessions are now available from the NRSP-6 Solanum germplasm collection.

B.  Preservation and Increase of Stocks

In 2001, 91 accessions were increased as botanical seed populations.

This year a total of 900 potato spindle tuber viroid (PSTV) tests were performed on seed increase parents, seed lots and research materials. Germination tests were performed on 1400 accessions; ploidy determinations were made on 325 accessions.

After last year’s limited success with spraying of 2,4-D on pollinated flowers to help in fruit retention and seed production, different chemicals are being studied to produce increased flowering and increased tuber initiation and production on wild potato species. 

The Association of Potato Inter-genebank Collaborators (APIC) constructed a database of all wild potato holdings of the potato genebanks in Argentina, Europe, Peru, and the United States.  The Inter-genebank Potato Database (IPD) is now being managed by Robert Hijmans at CIP (www.potgenebank.org/ipd/) and continues to be a very useful resource for clientele around the world.

C.  Classification

Dr. Spooner continues to resolve problems in taxonomic classification, which impede both efficient documentation and use of the germplasm. At present Dr. Spooner, in collaboration with Dr. R van den Berg, Dr. R. Hijmans, and Dr. A. Rodriguez, is working on a book of Wild Potato Species of North and Central America, which will be a valuable tool for germplasm users world wide.

D.  Distribution

NRSP-6 distributed 6,099 units of seed, 302 tuber families and 1,214 in vitro stocks to clientele in 20 states of the United States and 15 other countries.  Internally, NRSP-6 used 4,061 units of seed for chromosome counts, germination tests, identification and taxonomic check plantings, in vitro maintenance, seed increases, PSTV tests, and miscellaneous plantings.  The volume and types of stocks sent to various consignee categories are summarized in the table below.

VOLUME AND TYPES OF STOCKS DISTRIBUTED

¹ Types of stocks sent/(number of seeds, tubers or plantlets per standard shipping unit): S= True Seeds/(50),

  TF= Tuber Families/(10),  IVS=In Vitro Stocks/(1), FSG=Fine Screening Genotypes/(1).

² Includes chromosome counts, germination tests, ID and Taxonomic check plantings, in vitro maintenance, seed

  increases, PSTV tests, miscellaneous plantings and NSSL seed backup.

E.  Evaluation of Stocks

Mission

The project's mission with respect to evaluation is to locate and characterize useful traits so that the best materials and most efficient approaches are available for subsequent germplasm enhancement.

      1. Late Blight Screening

Breeding and genetic stocks were tested in Mexico; in addition, an inoculated test plot was initiated in an isolated field near the genebank. 

2. Antioxidant Screening  

A cooperative agreement was initiated with colleagues in Texas to test exotic species for high tuber antioxidants.  Species found to be promising in preliminary tests became the subject of more focused testing this year.

3. Glycoalkaloid Screening 

Tuber and leaf samples from families previously identified as having a combination of very high leaf and very low tuber glycoalkaloids were produced.  The present focus is on testing a greater number of individuals from a segregating family and intermating the best selected clones for recurrent selection for maximum leaf : tuber ratio.

4. Cold Genetics, Breeding and Physiology

Recurrent selection for good tuber characteristics in hybrids of tuberosum with extremely cold hardy species was continued.  A homemade freezing apparatus was used to test leaves from hundreds of segregating field-grown plants.  Some of these show great improvement in cold hardiness combined with good tuber type and earliness.

5. Tuber Calcium Accumulation

The segregation of an interspecific family between parents with extremely different  abilities of accumulating tuber calcium when low levels are available from the soil was tested.

6. Hormone Mutants

Plants carrying the ga1 mutant for response to exogenous GA3 in vitro were tested.  The mutant responds differently in different genetic backgrounds, suggesting some minor modifying genes may also be operating.

7. Apomixis.  DNA markers show preliminary evidence to support apomixis suspected in certain S. jamesii populations.  These preliminary results could have great application in potato breeding and germplasm preservation and could  reveal in situ population dynamics that impact collecting.

F.  Inter-genebank Collaboration

The Association of Potato Inter-genebank Collaborators (APIC) was formed in 1991 to assemble a database of potato germplasm available around the world and to initiate collaborative research projects. Below are some of the projects currently in progress.

1. Reputed duplicates in US and CIP (Peru) genebank with RAPDs.  Significant differences were less common than in previous comparisons between the VIR (Russian) and US genebanks.   A  collaboration with VIR on nematode screening of S. andigena accessions held at both genebanks was accomplished creating the possibility of comparing the presence of a practical trait in reputed duplicates in the two genebanks.

2. Import of rescued seeds from Poland.

Over 100 samples of unique VIR genebank accessions that were rescued (seed increased) under a special cooperative USDA/FAS project in Poland (coordinated by Chuck Brown) were imported.  Multiplication of these accessions has been initiated at the genebank.

3.  Collecting native US wild potatoes.

Collection continued at Southwest sites missed in previous expeditions.  Among the notable results of this trip, was the discovery of S. jamesii germplasm from a county in New Mexico from which wild potato has never been previously reported.

G.  Visitors From Other Countries

Dr. Y. Chen                    Taiwan

Dr. W. Roca                   Peru

Dr. S. Kiru                      Russia 

Dr. R. van den Berg        Netherlands

Dr. A. Rodriguez             Mexico

4.  USEFULNESS OF FINDINGS

NRSP-6's purpose is to provide a ready source of raw materials, technology and information to, support potato enhancement, breeding and research in the US and around the world.  Thus, one way the success of NRSP-6 can be measured is by the use of NRSP-6 germplasm in the pedigrees of new, improved potato cultivars.  Another is in the use of NRSP-6 stocks in more basic research programs, which also ultimately contribute to human utilization of the potato crop, these being reflected in publications.

Eleven cultivar releases were published in the American Journal of Potato Research in 2001: ‘AC Peregrine Red’, ‘AC Glacier Chip’, ‘AC Maple Gold’, ‘Amey’, ‘Eva’, ‘Jacqueline Lee’, ‘Klamath Russet’, ‘Liberator’, ‘Mazama’, ‘Michigan Purple’, and ‘Winema’.  All are known to have wild species in their pedigrees.

Section 6 lists 111 papers, 32 abstracts, and 5 theses which report the use of NRSP-6 Solanum introductions this year.

 5.  WORK PLANNED FOR 2002

Evaluation experiments will be continued on Solanum species for the following traits: antioxidants, frost hardiness, rooting vigor, tuber calcium, late blight resistance, hormone mutants and glycoalkaloids.

The general objective of NRSP-6 is to promote and facilitate potato research and breeding, will be pursued by continuing high quality service with respect to introduction, preservation, classification, evaluation, and distribution of potato germplasm to clients in the U.S. and around the world.

APIC inter-genebank projects researching the status and dynamics of genetic diversity in the collection using RAPDs and strengthening ties with sister genebanks around the world will be continued.

 6. PUBLICATIONS ISSUED DURING THE YEAR

A.     Publications issued by NRSP-6 Personnel

Bamberg, J.B., S.D. Kiru and A.H. del Rio.  2001.  Comparison of reputed duplicate populations in the Russian and US potato genebanks using RAPD markers.  Am. J. Potato Res. 78:365-369.

Bamberg, J.B., S. Love and D. Corsini.  2001.  Fine screening potato germplasm for high leaf and low tuber glycoalkaloids.  Am. J. Potato Res. 78:443.  (Abstract)

del Rio, A.H. and J.B. Bamberg.  2001.  Genetic heterogeneity among breeding systems of potato species and its ramifications in germplasm conservation.  Am. J. Potato Res. 78:452-453.  (Abstract)

del Rio, A.H., J.B. Bamberg, Z. Huaman, A. Salas, and S.E. Vega.  2001.  Association of ecogeographical variables and genetic variation in native wild US potato populations determined by RAPD markers.  Crop Science 41:870-878.

Douches, D.S., J.B. Bamberg, W. Kirk, K. Jastrzebski, B.A. Niemira, J. Coombs, D.A. Biognin, and K.J. Fletcher.  2001.  Evaluation of wild Solanum species for resistance to the US-8 genotype of Phytophthora infestans utilizing a fine-screening technique.  Am. J. Potato Res. 78:159-165.

Hale, Anna L., L. Cisneros-Zevallos, John B. Bamberg, and J. Creighton Miller, Jr.  2001.  Identification of named varieties, advanced selections and accessions with high antioxidant activity for use in breeding potatoes for enhanced human health benefits.  Am. J. Potato Res. 78:455-456.  (Abstract)

Kiru, S., S. Makovskaya and J. Bamberg.  2001.  Resistance of Solanum andigenum accessions maintained at VIR and US potato genebanks to Golden Nematode.  Am. J. Potato Res. 78:463.  (Abstract)

Lara-Cabrera, Sabina I. and David M. Spooner.  2001.  Taxonomy of Mexican diploid wild potato (Solanum sect. Petota) species: morphological and microsatellite data.  Am. J. Potato Res. 78:465-466.  (Abstract)

Hijmans, R. and D.M. Spooner.  2001.  Geographic distribution of wild potato species.  Amer. J. Bot. 88:2101-2122.

Peralta, E. and D.M. Spooner.  2001.  GBSSI Gene Phylogeny of Wild Tomatoes (Solanum L. section Lycopersicon [Mill.] Wettst. Subsection Lycopersicon).  Amer. J. Bot. 88:1888-1902.

Salas, A.R., D.M. Spooner, Z. Huaman, R.V. Torres-Maita, R. Hoekstra, K. Schuler, and R.J. Hijmans.  2001.  Taxonomy and new collections of wild potato species in central and southern Peru in 1999.  Am. J. Potato Res. 78:197-207.

Spooner, D.M.  2001.  Potato.  Encyclopedia of plant sciences, Ed. Richard Robinson, pp. 184-185.  Macmillan Reference USA, Gale Group, Farmington Hills, MI.

Spooner, D.M. and R. Hijmans.  2001.  Potato systematics and germplasm collecting, 1989-2000.  Am. J. Potato Res. 78:237-268; 395.

Spooner, D.M., R. Hoekstra and B. Vilchez.  2001.  Solanum sect. Petota in Costa Rica: taxonomy and genetic resources.  Am. J. Potato Res. 78:91-98.

Spooner, D. and S. Lara-Cabrera.  2001.  Sistematica molecular y evolucion de plantas cultivadas.  In: H.M. Hernandez, A. Garcia-Aldrete, F. Alvarez & M. Ulloa. (Eds.).  Enfoques Contemporaneos para el Estudio de la Biodiversidad.  Instituto de Biologia, UNAM/Fondo de Cultura Economica, Mexico, pp. 57-114.

Spooner, D.M., A. Salas and R. Hijmans.  2001.  Wild potato germplasm collecting expedition to Honduras and Panama.  Am. J. Potato Res. 78:485.  (Abstract)

Spooner, D.M. and R.G. van den Berg.  2001.  Quantitative assessment of corolla shape variation in Mexican Solanum sect. Petota. Pp. 61-71 In: van den Berg, R.G., G. Barendse, G.W. van der Weerden, and C. Mariani, (eds.).  Solanaceae V: Progress in taxonomy and utilization.  Nijmegen Univ. Press, Nijmegen, The Netherlands.

Spooner, D.M., R.G. van den Berg and J.T. Miller.  2001.  Species and series boundaries of Solanum series Longipedicellata (Solanaceae) and phonetically similar species in ser. Demissa and ser. Tuberosa: implications for a practical taxonomy of sect. Petota.  Amer. J. Bot. 88:113-130.

Spooner, D.M., R.G. van den Berg, A. Rivera-Pena, P. Velguth, A. del Rio, and A. Salas.  2001.  Taxonomy of Mexican and Central American members of Solanum Series Conicibaccata (sect. Petota) Syst. Bot. 26:743-756.

van den Berg, R.G., N. Groendijk-Wilders, M.J. Zevenbergen, and D.M. Spooner.  2001.  Molecular systematics of Solanum series Circaeifolia (Solanum section Petota) based on AFLP and RAPD markers. Pp. 72-84 In: van den Berg, R.G., G. Barendse, G.W. van der Weerden, and C. Mariani, (eds.).  Solanaceae V: Progress in taxonomy and utilization.  Nijmegen Univ. Press, Nijmegen, The Netherlands.

B.        Journal Articles and Abstracts Reporting Research with NRSP-6 Stocks

Anusuya Rangarajan, A.R. Miller and R.E. Veilleux.  2000.  Leptine glycoalkaloids reduce feeding by Colorado potato beetle in diploid Solanum sp. hybrids.  J. Am. Soc. Hort. Sci. 125(6):689-693.

Ashkenazi, V.E. Chani, U. Lavi, D. Levy, J. Hillel, and R.E. Veilleux.  2001.  Development of microsatellite markers in potato and their use in phylogenetic and fingerprinting analyses.  Genome 44:50-62.

Bachem, C.W.B., B. Horvath, L. Trindade, M. Claassens, E. Davelaar, W. Jordi, and R.G.F. Visser.  2001.  A potato tuber-expressed mRNA with homology to steroid dehydrogenases affects gibberellin levels and plant development.  Plant J. 25(6):595-604.

Barone, A., A. Sebastiano, D. Carputo, F. Della Rocca, and L. Frusciante.  2001.  Molecular marker-assisted introgression of the wild Solanum commersonii genome into the cultivated S. tuberosum gene pool.  Theor. Appl. Genet. 102(6/7):900-907.

Bastia, T., N. Carotenuto, B. Basile, A. Zoina, and T. Cardi.  2000.  Induction of novel organelle DNA variation and transfer of resistance to frost and Verticillium wilt in Solanum tuberosum through somatic hybridization with 1EBN S. commersonii.  Euphytica 116(1):1-10.

Bastia, T., N. Scotti and T. Cardi.  2001.  Organelle DNA analysis of Solanum and Brassica somatic hybrids by PCR with ‘universal primers’.  Theor. Appl. Genet. 102(8):1265-1272.

Beek, J.G. van der, P.W.T. Maas, G.J.W. Janssen, C. Zijlstra, and C.H. van Silfhout.  1999.  A pathotype system to describe intraspecific variation in pathogenicity of Meloidogyne chitwoodi.  J. Nematology 31(4):386-392.

Bink, M.C.A.M., P. Uimari, M.J. Sillanpaa, L.L.G. Janss, and R.C. Jansen.  2001.  Application of multiple QTL mapping in complex plant crosses to partial resistance to late blight (Phytophthora infestans) in potato: better insight by using a design-independent method.  In Quantitative genetics and breeding methods: the way ahead.  Proc. of 11^th Mtg. of EUCARPIA Sec. Biometrics in Plant Breeding, Paris, France, 8/30-9/1/00. [A. Gallais, C. Dillmann, I. Goldringer, (eds.).]

Bisognin, Dilson, Lynn Buszka and David Douches.  2001.  Mapping late blight resistance and other agronomic traits in a S. microdontum population.  Am. J. Potato Res. 78:444.  (Abstract)

Bradeen, James M., S. Kristine Naess, Susan M. Wielgus, Jeffrey A. Davis, Geraldine T. Haberlach, and John P. Helgeson.  2001.  Cloning late blight resistance from Solanum bulbocastanum.  Am. J. Potato Res. 78:445.  (Abstract)

Brodie, B.B.  1998.  Potato.  In: Plant and nematode interactions [K.R. Barker, G.A. Pederson, G.L. Windham, (eds.).].  Am. Soc. Agronomy, Madison, USA.

Brown, C.R., H. Mojtahedi and G.S. Santo.  1999.  Genetic analysis of resistance to Meloidogyne chitwoodi introgressed from Solanum hougasii into cultivated potato.  J. Nematology 31(3):264-271.

Carputo, D., A. Barone and L. Frusciante.  2000.  2n gametes in the potato: essential ingredients for breeding and germplasm transfer.  Theor. Appl. Genet. 101(5/6):805-8l3.

Carputo, D., B. Basile, T. Cardi, and L. Frusciante.  2000.  Erwinia resistance in backcross progenies of Solanum tuberosum x S. tarijense and S. tuberosum (+) S. commersonii hybrids.  Potato Res. 43(2):135-142.

Carrasco, A., J.I. Ruiz de Galarreta, A. Rico, and E. Ritter.  2000.  Transfer of PLRV resistance from Solanum verrucosum Schlechdt to potato (S. tuberosum L.) by protoplast electrofusion.  Potato Res. 43(1):31-42.

Christ, B.J.  2001.  Powdery Scab: An emerging disease on potato.  Am. J. Potato Res. 78:447-448.  (Abstract)

Christ, B.J. and K.G. Haynes.  2001.  Inheritance of resistance to early blight disease in a diploid potato population.  Plant Breeding 120(2):169-172.

Collins, W.  2000.  Research strategies for potatoes: a global approach.  In World Potato Congress: Proc. of Fourth World Potato Congress, Amsterdam, The Netherlands, Sept. 4-6, 2000.  Wageningen, Netherlands; Wageningen Pers 209-214.

Coombs, Joseph, David Douches, Edward Grafius, Walter Pett, and Dale Moyer.  2001.  Field evaluation of natural and engineered potato (Solanum tuberosum L.) resistance mechanisms for control of Colorado potato beetle (Leptinotarsa decemlineata Say).  Am. J. Potato Res. 78:448-449.  (Abstract)

Corsini, Dennis, Richard Novy, Annie Marshall, and Joseph Pavek.  2001.  Protein levels in potato breeding selections and cultivars.  Am. J. Potato Res. 78:449.  (Abstract)

Debnath, M.C., J.N. Khaund, B.K. Bora, and P.C. Sarmah.  2000.  The reaction of host plants on the biology of potato tuber moth, Phthorimaea operculella (Zeller).  Res. On Crops 1(2):196-200.

DeJong, H., K.G. Proudfoot and A.M. Murphy.  2001.  The germplasm release of F87084, a fertile, adapted clone with multiple disease resistances.  Am. J. Potato Res. 78:141-149.

DeJong, H., L.M. Kalwchuk, W.K. Coleman, C.A. Verhaeghe, L. Russell, V.J. Burns, and E. Tremblay-Deveau.  2001.  Development and characterization of an adapted form of Droopy, a diploid potato mutant deficient in abscisic acid.  Am. J. Potato Res. 78:279-290.

DeJong, H., L.M. Kawchuk, W.K. Coleman, C.A. Verhaeghe, L. Russell, V.J. Burns, and E. Tremblay-Deveau.  2001.  Development and characterization of an adapted form of Droopy, a diploid potato mutant deficient in abscisic acid.  Am. J. Potato Res. 78:451.  (Abstract)

Derie, M.L. and D.A. Inglis.  2001.  Persistence of complex virulences in populations of Phytophthora infestans in Western Washington.  Phytopathology 91(6):606-612.

Devine, K.J. and P.W. Jones.  2001.  Potato cyst nematode hatching activity and hatching factors in inter-specific Solanum hybrids.  Nematology 3(2):141-149.

Dong, F., J. Song, S.K. Naess, J.P. Helgeson, C. Gebhardt, and J. Jiang.  2000.  Development and applications of a set of chromosome-specific cytogenetic DNA markers in potato.  Theor. Appl. Genet. 101(7):1001-1007.

Dong, F., J.M. McGrath, J.P. Helgeson, and J. Jiang.  2001.  The genetic identity of alien chromosomes in potato breeding lines revealed by sequential GISH and FISH analyses using chromosome-specific cytogenetic DNA markers.  Genome 44:729-734.

Dorrance, A.E., D.A. Inglis, J.P. Helgeson, and C.R. Brown.  2001.  Partial Resistance to Phytophthora infestans in Four Solanum Crosses.  Am. J. Potato Res. 78:9-17.

Douches, D.S., K. Jastrzebski, J. Coombs, W.W. Kirk, K.J. Felcher, R. Hammerschmidt, and R.W. Chase.  2001.  Jacqueline Lee: A late-blight-resistant tablestock variety.  Am. J. Potato Res. 78:413-419.

Douches, D.S., K.J. Felcher, J. Coombs, C. Long, R. Hammerschmidt, and W.W. Kirk.  2001.  Michigan Purple: A purple-skinned tablestock variety.  Am. J. Potato Res. 78:421-424.

Douches, D.S., K. Jastrzebski, J. Coombs, R.W. Chase, R. Hammerschmidt, and W.W. Kirk.  2001.  Liberator: A round white chip-processing variety with resistance to scab.  Am. J. Potato Res. 78:425-431.

Eijlander, R., W. ter Laak, J.G. Th. Hermsen, M.S. Ramanna, and E. Jacobsen.  2000.  Occurrence of self-compatibility, self-incompatibility and unilateral incompatibility after crossing diploid S. tuberosum (SI) with S. verrucosum (SC): I. Expression and inheritance of self-compatibility.  Euphytica 115(2):127-139.

Estrada, N., C.E. Nustez and Sonia Tinjaca.  2001.  La biodiversidad en el mejoramiento de la Papa I-Resistencia de la papa a la “gota” causada por el Phytophthora infestans.  Experiencias y avances en su hibridacion en Colombia.  Memorias VIII Reunion de la Sociedad Colombiana de Fitomejoramiento y Produccion de Cultivos-Ibague, Colombia.

Estrada, N. and C.E. Nustez.  2001.  La biodiversidad en el fitomejoramiento de la Papa II-Resistencia de la papa a las heladas.  Experiencias y avances en su hibridacion en Colombia.  Memorias VIII Reunion de la Sociedad Colombiana de Fitomejoramiento y Produccion de Cultivos-Ibague, Colombia.

Eusebio, J.E. and E.N. Bernardo.  1999.  Techniques of potato germplasm evaluation for broad mite (Polyphagotarsonemus latus (Banks)) resistance.  Philippine Entomologist 13(1):35-41.

Fernandez, E.C. and E.N. Bernardo.  1999.  Evaluation of potato (Solanum spp.) germplasm in the Philippines for resistance to thrips, Thrips palmi Karny.  Philippine Entomologist 13(1):21-34.

Flier, W.G., N.J. Grunwald, W.E. Fry, and L.J. Turkensteen.  2001.  Formation, production and viability of oospores of Phytophthora infestans from potato and Solanum demissum in the Toluca Valley, central Mexico.  Mycological Res. 105(8):998-1006.

Fock, I., C. Collonnier, A. Purwito, J. Luisetti, V. Souvannavong, F. Vedel, A. Servaes, A. Ambroise, H. Kodja, G. Ducreux, and D. Sihachakr.  2000.  Resistance to bacterial wilt in somatic hybrids between Solanum tuberosum and Solanum phureja.  Plant Science 160(1):165-176.

Gao Gang, Qu DongYu, Lian Yong, Jin LiPing, and Feng LanXiang.  2000.  Identification molecular markers linked with resistance to bacterial wilt (Ralstonia solanacearum) in diploid potato.  Acta Hort. Sinica 27(1):37-41.

Gavrilenko, T., R. Rhieme and V.M. Rokka.  2001.  Cytogenetic analysis of Lycopersicon esculentum (+) Solanum etuberosum somatic hybrids and their androgenetic regenerants.  Theor. Appl. Genet. 103(2/3):231-239.

Ghislain, M., B. Trognitz, M. del R. Herrera, J. Solis, G. Casallo, C. Vasquez, O. Hurtado, R. Castillo, L. Portal, and M. Orrillo.  2001.  Genetic loci associated with field resistance to late blight in offspring of Solanum phureja and S. tuberosum grown under short-day conditions.  Theor. Appl. Genet. 103(2/3):433-442.

Hamernik, A.J., J. Havey and R.E. Hanneman, Jr.  2001.  Wild species, nature’s treasure to potato improvement.  Proceedings of Wisconsin’s Annual Potato meetings 2001.

Hamernik, A.J., M. Ramon and R.E. Hanneman, Jr.  2001.  Modified conventional breeding methods to efficiently transfer unique late blight resistance from 2x(1EBN) Mexican species to 2x(2EBN) and 4x(4EBN) breeding lines.  Am. J. Potato Res. 78:456.  (Abstract)

Hanneman, R.E. Jr., A.J. Hamernik and M.J. Havey.  2001.  Evaluation of parental materials and enhancement selections for resistance to late blight, Colorado Potato Beetle, early dying and scab in field tests at the UW Agricultural Research Station, Hancock, Wisconsin.  K.G. Haynes (ed.).  National Potato Germplasm Evaluation and Enhancement Report, 2000.  USDA, ARS, Beltsville, Maryland.

Harding, K. and S. Millam.  2000.  Analysis of chromatin, nuclear DNA and organelle composition in somatic hybrids between Solanum tuberosum and  Solanum sanctae-rosae.  Theor. Appl. Genet. 101(5/6):939-947.

Hayes, Ryan J. and C.A. Thill.  2001.  Identification of 4C chipping in 12 late blight resistance Solanum species.  Am. J. Potato Res. 78:457.  (Abstract)

Hayes, Ryan J. and C.A. Thill.  2001.  Co-current introgression of economically important traits in a potato breeding program.  Am. J. Potato Res. (In press).

Hayes, Ryan J. and C.A. Thill.  2001.  Sexual polyploidization in breeding for cold chipping potato cultivars.  ASA:(Abstract, in press).

Hayes, Ryan J. and C.A. Thill.  2001.  Co-current introgression of economically important traits from wild to cultivated potato.  ASA:(Abstract, in press).

Haynes, K.G., G.A. Porter, B.J. Christ, R.W. Goth, K.O. DeLong, D.E. Halseth, J.B. Sieczka, M.R. Henninger, S.B. Sterrett, G.C. Yencho, and R.E. Webb.  2001.  Amey: A multipurpose, russet-skinned potato cultivar for the Eastern United States.  Am. J. Potato Res. 78:175-181.

Haynes, Kathleen G.  2001.  Variance components for yield and specific gravity in a diploid potato population after two cycles of recurrent selection.  Am. J. Potato Res. 78:69-75.

Haynes, Kathy G., C.A. Thill, G. Secor, W.E. Fry, D.S. Douches, W.R. Stevenson, and D.P. Weingartner.  2001.  Foliar resistance to late blight in potato clones evaluated in national trials in 2000.  Am. J. Potato Res. 78:(In press).

Hijmans, R.J., K.A. Garrett, Z. Huaman, D.P. Zhang, M. Schreuder, and M. Bonierbale.  2000.  Assessing the geographic representativeness of genebank collections: the case of Bolivian wild potatoes.  Conservation Bio. 14(6):1755-1765.

Horsman, K., T. Gavrilenko, M. Bergervoet, D.J. Huigen, A.T.W. Joe, and E. Jacobsen.  2001.  Alteration of the genomic composition of Solanum nigrum (+) potato backcross derivatives by somatic hybridization: selection of fusion hybrids by DNA measurements and GISH.  Plant Breed. 120(3):201-207.

Hosaka, Kazuyoshi, Yuko Hosaka, Motoyuki Mori, Tsutomu Maida, and Hiroshi Matsunaga.  2001.  Detection of a simplex RAPD marker linked to resistance to potato virus Y in a tetraploid potato.  Am. J. Potato Res. 78:191-196.

Hutvagner, G., Z. Banfalvi, I. Milankovics, D. Silhavy, Z. Polgar, S. Horvath, P. Wolters, and J.P. Nap.  2001.  Molecular markers associated with leptinine production are located on chromosome 1 in Solanum chacoense.  Theor. Appl. Genet. 102(6/7):1065-1071.

Jansen, G., W. Flamme, K. Schuler, and M. Vandrey.  2001.  Raw material and starch quality of wild and cultivated potato species and varieties.  Potato Res. 44:137-146.

Jansky, S.H. and D.I. Rouse.  2000.  Identification of potato interspecific hybrids resistant to Verticillium wilt and determination of criteria for resistance assessment.  Potato Res. 43(3):239-251.

Johnson, A.A.T., S.M. Piovano, V. Ravichandran, and R.E. Veilleux.  2001.  Selection of monoploids for protoplast fusion and generation of intermonoploid somatic hybrids of potato.  Am. J. Potato Res. 78:19-29.

Johnson, A.A.T., B.A. Nault and R.E. Veilleux.  2001.  Transgenic resistance to Colorado potato beetle (Leptinotarsa decemlineata) transmitted to tetraploid potato through 4x-2x hybridization.  Am. J. Potato Res. 78:461.  (Abstract)

Kormut’ak, A., J. Heldak and D. Subova.  1999.  Soluble proteins and isoesterases as taxonomic markers tested on nine wild Solanum species and eight Slovakian potato varieties.  In: Proc. of conference on potato seed production by tissue culture, Brussels, Belgium, Feb. 25-28, 1998.  Potato Res. 42(3/4):619-626.

Kozlov, V.A.  2000.  Using the species Solanum andigenum for creating potato lines resistant to Phytophthora blight.  Vestsi Akad. Agr. Navuk Res. Belarus No. 4, 52-56.

Kuhl, J.C., R.E. Hanneman, Jr. and M.J. Havey.  2001.  Characterization and mapping of Rpil, a late blight resistance locus from diploid (1EBN) Mexican Solanum pinnatisectum.  Molec. Genet. Genomics 265:977-985.

Kuhl, J.C., M.J. Havey and R.E. Hanneman, Jr.  2001.  Genetic studies of unilateral incompatibility between diploid (1EBN) Mexican species Solanum pinnatisectum and S. cardiophyllum subsp. cardiophyllum.  Am. J. Potato Res. 78:464-465.  (Abstract)

Lai, A., V. Cianciolo, S. Chiavarini, and A. Sonnino.  2000.  Effects of glandular trichomes on the development of Phytophthora infestans infection in potato (s. tuberosum).  Euphytica 114(3):165-174.

Lees, A.K., M.J. de Maine, M.J. Nicolson, and J.E. Bradshaw.  2000.  Long-day-adapted Solanum phureja as a source of resistance to blackleg caused by Erwinia carotovora subsp. astroseptica.  Potato Res. 43(3):279-285.

Lennartsson, M., R. Manchett, C. Schauer, K. Schuler, R.J. Turner, R.M. Vogel, and B. Zuna-Kratky.  2000.  Characterization and utilization of secondary potato varieties for organic farming.  In IFOAM 2000: the world grows organic. Proc. 13^th International IFOAM Scientific Conference, Basel, Switzerland, 28-3l August, 2000.  T. Alfoldi, W. Lockeretz, and U. Niggli (eds.).

Levy, D., Y. Itzhak, E. Fogelman, E. Margalit, and R.E. Veilleux.  2001.  Ori, Idit, Zohar, and Zahov: tablestock and chipstock cultivars bred for adaptation for Israel.  Am. J. Potato Res. 78:167-173.

Lightbourn, G. and R.E. Veilleux.  2001.  Ploidy reduction of a fertile tetraploid intermonoploid somatic hybrid for studying somaclonal variation.  Am. J. Potato Res. 78:466.  (Abstract)

Lorenzen, J.H., N.F. Balbyshev, A.M. Lafta, H. Casper, X. Tian, and B. Sagredo.  2001.  Resistant potato selections contain leptine and inhibit development of the Colorado potato beetle (Coleoptera: Chrysomelidae).  J. Econ. Entomol. 94:1260-1267.

Lough, R.C., J.M. Varrieur and R.E. Veilleux.  2001.  Selection inherent in monoploid derivation mechanisms for potato.  Theor. Appl. Genet. 103:178-184.

Love, Stephen L., Thomas A. Salaiz, Joseph J. Pavek, and Charles R. Brown.  2001.  Development of russet-type germplasm with resistance to Corky ringspot.  Am. J. Potato Res. 78:467-468.  (Abstract)

Luu DoanTrung, Qin Xike, D. Morse, and M. Cappadocia.  2000.  S-Rnase uptake by compatible pollen tubes in gametophytic self-incompatibility.  Nature 407(6804):649-651.

Lynch, D.R., S.J. Peloquin, L.M. Kawchuk, C.A. Schaupmeyer, J. Holley, D.K. Fujimoto, D. Driedger, D. Waterer, J. Wahab, and M.S. Goettel.  2001.  AC Glacier Chip: A high-yielding chip cultivar for long-term storage.  Am. J. Potato Res. 78:327-332.

Lynch, D.R., G. Secor, L.M. Kawchuk, D. Waterer, C.A. Schaupmeyer, J. Holley, D.K. Fujimoto, D. Driedger, J. Wahab, and M.S. Goettel.  2001.  AC Peregrine Red: A high-yielding red-skinned fresh market cultivar.  Am. J. Potato Res. 78:333-337.

Lynch, D.R., L.M. Kawchuk, C.A. Schaupmeyer, J. Holley, D.K. Fujimoto, D. Driedger, T.R. Tarn, D. Waterer, J. Wahab, and M.S. Goettel.  2001.  AC Maple Gold: A high-yielding yellow-fleshed french fry cultivar.  Am. J. Potato Res. 78:339-343.

Martinez, C.A., M.E. Loureiro, M.A. Oliva, and M. Maestri.  2001.  Differential responses of superoxide dismutase in freezing resistant Solanum curtilobum and freezing sensitive Solanum tuberosum subjected to oxidative and water stress.  Plant Sci. 160(3):505-515.

Masuelli, R.W.  2001.  Mitotic-cycle time and the development of embryo and endosperm in compatible and incompatible crosses in tuber-bearing Solanum species.  Genome 44(3):426-431.

Micheletto, S., R. Boland and M. Huarte.  2000.  Argentinian wild diploid Solanum species as sources of quantitative late blight resistance.  Theor. Appl. Genet. 101(5/6):902-906.

Molnar, A., A. Lovas, Z. Banfalvi, L. Lakatos, Z. Polgar, and S. Horvath.  2001.  Tissue-specific signal(s) activate the promoter of a metallocarboxypeptidase inhibitor gene family in potato tuber and berry.  Plant Mol. Bio. 46(3):301-311.

Mosley, A.R., K.A. Rykbost, S.R. James, D.C. Hane, C.C. Shock, B.A. Charlton, J.J. Pavek, S.L. Love, D.L. Corsini, and R.E. Thornton.  2001.  Klamath Russet: A full season, fresh market, long Russet.  Am. J. Potato Res. 78:377-381.

Naess, S.K., J.M. Bradeen, S.M. Wielgus, G.T. Haberlach, J.M. McGrath, and J.P. Helgeson.  2000.  Resistance to late blight in Solanum bulbocastanum is mapped to chromosome 8.  Theor. Appl. Genet. 101(5/6):697-704.

Naess, S.K., J.M. Bradeen, S.M. Wielgus, G.T. Haberlach, J.M. McGrath, and J.P. Helgeson.  2001.  Analysis of the introgression of Solanum bulbocastanum DNA into potato breeding lines.  Mol. Genet. Genom. 265(4):694-704.

Naess, S.K., J.M. Bradeen, J. Song, G.T. Haberlach, S.M. Wielgus, J.A. Davis, J. Jiang, and J.P. Helgeson.  2001.  Accelerating the chromosome walk towards late blight resistance with the help of FISH.  Am. J. Potato Res. 78:471.  (Abstract)

Nakagawa, K., H. Uehara and K. Hosaka.  2000.  Chloroplast DNA variation in the wild potato species, Solanum acaule and S. albicans.  Euphytica 116(3):197-202.

Novy, Richard, Dennis Corsini, Joe Pavek, Hector Lozoya-Saldana, and Alejandro Hernandez-Vilchis.  2001.  Analysis of segregation for late blight resistance in potato families screened at Toluca Valley, Mexico.  Am. J. Potato Res. 78:474.  (Abstract)

Nustez, C.E., N. Estrada, Celsa Garcia, Sonia Tinjaca, J.P. Medina, and Schilde Lieselote.  2001.  Evaluacion de la resistencia a P. infestans, cruzabilidad y potencial de rendimiento de 15 clones de hibridacion somatica de Solanum bulbocastanum + S. phureja en Colombia.  Actas p. 427-434.  Vitoria Gasteiz, España.

Oberwalder, B., L. Schilde-Rentschler, B. Loffelhardt-Ruoss, and H. Ninnemann.  2000.  Differences between hybrids of Solanum tuberosum L. and  Solanum circaeifolium Bitt. obtained from symmetric and asymmetric fusion experiments.  Potato Res. 43(1):71-82.

Ochoa, C. and D. Ugent.  2000.  Solanum hygrothermicum, an endangered cultivated potato species.  Economic Botany 54(2):228.

Pavek, J.J. and D.L. Corsini.  2001.  Utilization of potato genetic resources in variety development.  Am. J. Potato Res. 78:433-441.

Pelletier, Y., C. Clark and G.C. Tai.  2001.  Resistance of three wild tuber-bearing potatoes to the Colorado potato beetle.  Ent. Exp. Appl. 100(1):31-41. 

Petr, P. and J. Pavlas. 2000.   Possibility of use of South American sources of field resistance to Phytophthora infestans in potato breeding in the Czech Republic.  Czech J. Genet. Pl. Breed. 36(2):59-65.

Petukhov, S.N. and S.M. Musin.  2001.  Prospects for the use of wild diploids and dihaploids in potato breeding.  Kartofel’ i Ovoshchi No. 3, 41.

Plaisted, R.L., D.E. Halseth, B.B. Brodie, S.A. Slack, J.B. Sieczka, B.J. Christ, K.M. Paddock, and M.W. Peck.  2001.  Eva: A midseason golden nematode- and virus-resistant variety for use as tablestock or chipstock.  Am. J. Potato Res. 78:65-68.

Posch, Danielle M. and C.A. Thill.  2001.  Identifying resistance to Phytophthora infestans earlier in breeding.  Am. J. Potato Res. 78:In Press.  (Abstract)

Qin, X., D.T. Luu, Q. Yang, O. Maes, D.P. Matton, D. Morse, and M. Cappadocia.  2001.  Genotype-dependent differences in S₁₂-RNase expression lead to sporadic self-compatibility.  Pl. Mol. Bio. 45(3):295-305.

Rogan, G.J., J.T. Bookout, D.R. Duncan, R.L. Fuchs, P.B. Lavrik, S.L. Love, M. Mueth, T. Olson, E.D. Owens, P.J. Raymond, and J. Zalewski.  2000.  Compositional analysis of tubers from insect and virus resistant potato plants.  J. Ag. Food Chem. 48(12):5936-5945.

Rojas-Cifuentes, G.A., P.M.A. Kianian and R.G. Novy.  2000.  Potato RAPD heteroduplexes: Their impact on genetic distance estimates and their use in developing codominant sequence tagged site (STS) markers.  Am. J. Potato Res. 77:416-417.  (Abstract)

Rorat, T.  2001.  Changes in gene expression in wild potato (Solanum sogarandinum) during cold acclimation.  Acta Phys. Plant. 23(1):117-126.

Rykbost, K.A., R. Voss, S.R. James, A.R. Mosley, B.A. Charlton, D.C. Hane, R.H. Johansen, S.L. Love, and R.E. Thornton.  2001.  Winema: An early maturing, red-skinned cultivar for fresh market.  Am. J. Potato Res. 78:371-375.

Rykbost, K.A., R. Voss, S.R. James, A.R. Mosley, B.A. Charlton, D.C. Hane, R.H. Johansen, S.L. Love, and R.E. Thornton.  2001.  Mazama: An early maturing, bright red-skinned cultivar for fresh market.  Am. J. Potato Res. 78:383-387.

Sagredo B., A. Lafta, A. Casper, J.H. Lorenzen.  2001.  Identification and genetic location of a novel potato alkaloid.  Annual meeting.  Potato Association of America.  (Abstract)

Sagredo B., A. Lafta, A. Casper, J.H. Lorenzen.  2001.  AFLP mapping of genes controlling leptine synthesis in tetraploid potatoes.  Annual meeting.  Potato Association of America.  (Abstract)

Saha, M.C. and J.H. Lorenzen.  2001.  Mapping of genes associated to late blight, Verticillium wilt and cold-sweetening resistance in potatoes.  Am. J. Potato Res. 78:480.  (Abstract)

Schüler, K.  2001.  Solanum tuberosum (potatoes).  Paper for Encyclopedia of Genetics, Herausgeber S. Brenner, J.H. Miller u.a., Academic Press.

Schüler, K.  2001.  Bericht über die Kartoffelsammelexpedition in Peru 1999.  Schriftenreihe zu genetischen Ressourcen, Band 16: Nutzung genetischer Ressources- ökologischer Wert der Biodiversität, ZADI Bonn.

Schüler, K.  2001.  Status of potato genetic resources and research in Germany.  In: R. Hoekstra, L. Maggioni, E. Lipman: Report of a Working Group on Potato, Wageningen, The Netherlands, 23-25 November 2000, IPGRI Rome, pp 26-32.

Seabrook, J.E.A. and L.K. Douglass.  2001.  Somatic embryogenesis on various potato tissues from a range of genotypes and ploidy levels.  Plant Cell Reports. 20:175-182.

Silva, J.A.B., W.C. Otoni, C.A. Martinez, L.M. Dias, and M.A.P. Silva.  2001.  Microtuberization of Andean potato species (Solanum spp.) as affected by salinity.  Sci. Hort. 89(2):91-101.

Sowokinos, Joseph R.  2001.  Invited Review: Biochemical and molecular control of cold-induced sweetening in potatoes.  Am. J. Potato Res. 78:221-236.

Stein, C.P. and J.D. Vendramim.  2000.  Antibiosis of potato clones to Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae).  Anais da Soc. Entom. do Brasil 29(4):783-788.

Sterrett, S.B., M.R. Henninger, G.C. Yencho, and K.G. Haynes.  2001.  S. phureja-S. stenotomum contributes high specific gravity and internal tuber quality under high temperature growing environments to 4x-2x hybrids.  Am. J. Potato Res. 78:485.  (Abstract)

Tai, George C.C., Henry DeJong, T. Richard Tarn, and Warren K. Coleman.  2001.  Variation of chipping quality in three genetic populations derived from tuberosum x tuberosum, andigena x tuberosum and diploid x diploid crosses.  Am. J. Potato Res. 78:486.  (Abstract)

Thill, Christian A.  2001.  Minnesota potato breeding program.  Proc. National Potato Germplasm Evaluation and Enhancement Report, 2001.  72^nd Annual Report by Cooperators.  USDA/ARS.  K.G. Haynes (ed.).

Thill, Christian A.  2001.  NCR-84 potato variety trials.  Proc. National Potato Germplasm Evaluation and Enhancement Report, 2001.  72^nd Annual Report by Cooperators.  USDA/ARS.  K.G. Haynes (ed.).

Varrieur, J.M. and R.E. Veilleux.  2001.  Marker analysis of monoploid potato.  Am. J. Potato Res.  78:488-489.  (Abstract)

Vreugdenhil, D. and L.I. Sergeeva.  1999.  Gibberellins and tuberization in potato.  In Proc. of conference on potato seed production by tissue culture, Brussels, Belgium, Feb. 25-28, 1998.  Potato Res. 42(3/4):471-481.

Yencho, G. Craig, Mark Clough and Robert Goth.  2001.  Sucrose esters derived from Solanum berthaultii and resistance to potato late blight.  Am. J. Potato Res. 78:489.  (Abstract)

Young, L.D.  1998.  Breeding for nematode resistance and tolerance.  In: Plant and nematode interactions [K.R. Barker, G.A. Pederson, G.L. Windham, (eds.)].  Am. Soc. Of Agronomy, Madison, USA.

Zimnoch-Guzowska, E., W. Marczewski, R. Lebecka, B. Flis, R. Schafer-Pregl, F. Salamini, and C. Gebhardt.  2000.  QTL analysis of new sources of resistance to Erwinia carotovora ssp. atroseptica in potato done by AFLP, RFLP, and resistance-gene-like markers.  Crop Sci. 40(4):1156-1167.

Zlesak, David C. and C.A. Thill.  2001.  Variation for 2n pollen production and male fertility in wild Solanum germplasm resistant to Phytophthora infestans (Mont.) de Bary (US8).  Am. J. Potato Res. (In review).

Zlesak, David C. and C.A. Thill.  2001.  Obtaining sexual hybrids between Solanum pinnatisectum (1EBN) and cultivated potato germplasm.  Am. J. Potato Res. 78:489-490.  (Abstract)

C.        Theses Reporting Research with NRSP-6 Stocks

Hayes, Ryan J.  2001.  Accelerated development of cold (4C) chipping potatoes through early generation selection in diverse potato progenies.  University of Minnesota, M.S. Thesis, pp. 116.

Horsman, K.  2001.  Somatic hybrids of Solanum tuberosum and species of the Solanum nigrum-complex and their backcross progeny.  Thesis, Wageningen University, Wageningen, The Netherlands, 104 pp.

Johnson, A.A.T.  2001.  Effect of ploidy elevation, copy number and parent-of-origin on transgene expression in potato.  Ph.D. Dissertation, Virginia Polytechnic Institute & State University, Blacksburg, Virginia.

Rojas-Cifuentes, G.  2001.  RAPD heteroduplex formation in potato and its impact on genetic distance estimates.  Ph.D. Dissertation, North Dakota State University, Fargo, ND.

Zlesak, David C.  2001.  Identification and introgression of resistance to Phytophthora infestans (Mont.) de Bary (US8) from wild Solanum species into cultivated potatoes.  University of Minnesota, M.S. Thesis, pp. 155.

7.  APROVED

          _____________________________________________                    __________

          J. C. Miller, Jr., Chairman, Technical Committee                                      Date

          _____________________________________________                    __________

          P. W. Ludden, Lead Administrative Advisor                                            Date